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Catalog Number Size Price
LS-B16259-100 100 µg (200 µg/ml) $485 
NKX2-1 / Thyroid-Specific TF Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
NKX2-1 / Thyroid-Specific TF Antibody - SDS-PAGE Analysis Purified TTF-1 Mouse Recombinant Monoclonal Antibody (rNX2.1/690). Confirmation of Purity and Integrity of Antibody.
NKX2-1 / Thyroid-Specific TF Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
NKX2-1 / Thyroid-Specific TF Antibody - SDS-PAGE Analysis Purified TTF-1 Mouse Recombinant Monoclonal Antibody (rNX2.1/690). Confirmation of Purity and Integrity of Antibody.
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PathPlus™ Recombinant Monoclonal Mouse anti‑Human NKX2‑1 / Thyroid‑Specific TF Antibody (clone rNX2.1/690, IHC) LS‑B16259

PathPlus™ Recombinant Monoclonal Mouse anti‑Human NKX2‑1 / Thyroid‑Specific TF Antibody (clone rNX2.1/690, IHC) LS‑B16259

Note: This antibody replaces LS-C751392
Antibody:
NKX2-1 / Thyroid-Specific TF Mouse anti-Human Recombinant Monoclonal (rNX2.1/690) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B16259-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NKX2-1 / Thyroid-Specific TF Mouse anti-Human Recombinant Monoclonal (rNX2.1/690) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NKX2-1 (also known as thyroid transcription factor TTF-1) is a nuclear protein involved in the differentiation of lung, thyroid and forebrain cells. It regulates the expression of surfactant proteins in the lung that are required for host defense and stability. TTF-1 is a useful marker for distinguishing a primary lung adenocarcinoma from metastases, particularly when combined with Napsin A in a marker panel. In normal tissues, it is expressed in Type II pneumocytes, Clara Cells, thyroid follicular epithelium and parafollicular C cells. NKX2-1 antibodies are useful for distinguishing primary pulmonary adenocarcinoma (positive staining) from metastases, pleural lung carcinomas (positive) from mesotheliomas, and also small cell carcinoma of the lung (positive) from Merkel cell carcinoma. NKX2-1 is positive in small cell lung carcinomas, adenocarcinomas, thyroid hyperplasias and carcinomas, and neoplasias. It is expected to have nuclear staining. References: Clin Sci. 2009 Jan. 116.1; 27-35, PMID: 19037882; Clin Onc. 2009 Jan. 27.2; 271-278, DOI:10.1200/JCO.2008.17.0043; AIMM 2002 10(2):97; Annals of Oncology 2006 17(11):1672; Am J Surg path 2001 25:363; Mod path 2000 13:570
Target
Human NKX2-1 / Thyroid-Specific TF
Synonyms
NKX2-1 | BCH | Benign chorea | Homeobox protein Nkx-2.1 | NK-2 | NKX2.1 | NK-2 homolog A | Thyroid transcription factor 1 | NK2 homeobox 1 | TTF-1 | BHC | Thyroid nuclear factor 1 | TITF1 | NKX2A | TEBP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Recombinant Monoclonal
Clone
rNX2.1/690
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human TTF-1 protein. Antigen Molecular Weight: 40 kDa
Specificity
Recognizes a protein of 40 kDa , identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
Applications
  • IHC - Paraffin (1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NKX2-1 / Thyroid-Specific TF
P43699 NM_003317 NP_003308.1

Validation

NKX2-1 / Thyroid-Specific TF Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ NKX2-1 / Thyroid-Specific TF Antibody (clone rNX2.1/690) for IHC LS-B16259 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/27/2021