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Catalog Number Size Price
LS-B16308-100 100 µg (200 µg/ml) $485 
NF-L / NEFL Antibody - Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
NF-L / NEFL Antibody - Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
NF-L / NEFL Antibody - SDS-PAGE Analysis Purified Neurofilament Rabbit Monoclonal Antibody (NEFL/2983R). Confirmation of Purity and Integrity of Antibody.
NF-L / NEFL Antibody - Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
NF-L / NEFL Antibody - Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
NF-L / NEFL Antibody - SDS-PAGE Analysis Purified Neurofilament Rabbit Monoclonal Antibody (NEFL/2983R). Confirmation of Purity and Integrity of Antibody.
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PathPlus™ Recombinant Monoclonal Rabbit anti‑Human NF‑L / NEFL Antibody (clone NEFL/2983R, IHC) LS‑B16308

PathPlus™ Recombinant Monoclonal Rabbit anti‑Human NF‑L / NEFL Antibody (clone NEFL/2983R, IHC) LS‑B16308

Note: This antibody replaces LS-C751296
Antibody:
NF-L / NEFL Rabbit anti-Human Recombinant Monoclonal (NEFL/2983R) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B16308-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NF-L / NEFL Rabbit anti-Human Recombinant Monoclonal (NEFL/2983R) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NEFL refers to the neurofilament light polypeptide, a low molecular weight neurofilament protein. Neurofilaments are intermediate filaments that form the neuronal cytoskeleton together with microtubules. They act as structural space-fillers for axons and are also involved in axonal transport. Antibodies that detect NEFL are regularly used to identify neurons and distinguish them from glia (neurofilament-negative) and for diagnosis in neuropathology. Because neurofilaments are released into the blood and cerebrospinal fluid when axons and neurons deteriorate, antibodies can also be used to observe the progression and state of neurodegenerative diseases such as multiple sclerosis and Huntington’s disease. NEFL is also specifically implicated in Charcot-Marie-Tooth disease, described by a mutation-induced knockout of the gene that results in childhood-onset neuropathy. In immunohistochemistry, NEFL antibodies are positive in axons of neuronal cells in the central nervous system and in peripheral nerves. References: European Journal of Neurology. 2010 Mar. 17 (3): 377–8, PMID: 19845747; Neurology. 2009 Apr. 72 (15): 1322–9, PMID: 19365053; PLOS One. 2017 Feb. 12 (2): e0172762, PMID: 28241046; Garland Science. ISBN: 978-0-8153-3218-3; Neurology Genetics. 2018 June; 4 (3), DOI: 10.1212/NXG.0000000000000244; Brain. 2003 Mar. Volume 126, Issue 3; 590–597, DOI: 10.1093/brain/awg059
Target
Human NF-L / NEFL
Synonyms
NEFL | 68 kDa neurofilament protein | CMT1F | CMT2E | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Recombinant Monoclonal
Clone
NEFL/2983R
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human NEFL protein. Antigen Molecular Weight: 68 kDa
Specificity
This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Applications
  • IHC - Paraffin (1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistology (Formalin-fixed - 0.25-0.5 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NF-L / NEFL
P07196 NM_006158 NP_006149.2

Validation

NF-L / NEFL Antibody - Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

NF-L / NEFL Antibody - Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ NF-L / NEFL Antibody (clone NEFL/2983R) for IHC LS-B16308 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/17/2021