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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1753-50 | 50 µg (0.5 mg/ml) | $485 |
PathPlus™ Polyclonal Goat anti‑Human NET1 / ARHGEF8 Antibody (aa396‑409, IHC) LS‑B1753
PathPlus™ Polyclonal Goat anti‑Human NET1 / ARHGEF8 Antibody (aa396‑409, IHC) LS‑B1753
Note: This antibody replaces LS-C47574, LS-C73334
Antibody:
NET1 / ARHGEF8 Goat anti-Human Polyclonal (aa396-409) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NET1 / ARHGEF8 Goat anti-Human Polyclonal (aa396-409) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
NET1 (Neuroepithelial cell-transforming gene 1, Net1A, ARHGEF8) is a guanine nucleotide exchange factor protein that activates RhoA GTPase. Studies in zebrafish have illustrated that NET1 is important for embryogenesis and in establishing the body plan. Furthermore, variants in NET1 are associated with oppositional defiant disorder (ODD) and attention-deficit/hyperactivity disorder (ADHD) in humans. In cancer, deletion mutations in NET1 cause the protein to be re-localized to the cytosol, where it aberrantly activates RhoA and induces transformation. In immunohistochemistry, NET1 has cytoplasmic positivity in the brain, gastrointestinal tract, muscle, reproductive tissues, kidney, liver and some immune tissues.
References: J Child Psychol Psychiatry. 2015 Jan;56(1):58-66, PMID: 24942521; Zhonghua Yi Xue Za Zhi. 2015 Nov 3;95(41):3351-6, PMID: 26812975; Cell Res. 2017 Feb; 27(2): 202–225, PMID: 27910850; Mol Cell Biol. 2007 Dec; 27(24): 8683–8697, PMID: 17938206; Journal of Cell Science 2018 131: jcs204644, DOI: 10.1242/jcs.204644
Target
Human NET1 / ARHGEF8
Synonyms
NET1 | ARHGEF8 | NET1A | Proto-oncogene p65 Net1
Host
Goat
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2NP_001040625.1.
Epitope
aa396-409
Specificity
Human NET1. This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1753 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1753 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NET1 / ARHGEF8
Validation
Anti-NET1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NET1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ NET1 / ARHGEF8 Antibody (aa396-409) for IHC LS-B1753 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Reactivity:
Human
Range:
15.6-1000 pg/ml
Reactivity:
Human
Range:
0.41-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
