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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16286-100 | 100 µg (200 mg/ml) | $485 |
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PathPlus™ Recombinant Monoclonal Rabbit anti‑Human KRT18 / CK18 / Cytokeratin 18 Antibody (clone KRT18/2808R, IHC, IF, WB) LS‑B16286
PathPlus™ Recombinant Monoclonal Rabbit anti‑Human KRT18 / CK18 / Cytokeratin 18 Antibody (clone KRT18/2808R, IHC, IF, WB) LS‑B16286
Note: This antibody replaces LS-C751239
Antibody:
KRT18 / CK18 / Cytokeratin 18 Rabbit anti-Human Recombinant Monoclonal (KRT18/2808R) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
KRT18 / CK18 / Cytokeratin 18 Rabbit anti-Human Recombinant Monoclonal (KRT18/2808R) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
Cytokeratin 18 (KRT18, CK18) is a Type I intermediate filament chain keratin that is expressed in simple non-stratified epithelial tissues, eccrine glands, trophoblasts and endothelia of veins and lymphatics. Along with KRT8, KRT18 protects the liver from stress-induced injury and apoptosis. Mutations in this gene have been linked to cryptogenic cirrhosis and a variety of liver diseases, particularly after alcohol or viral infections. It is also a serum marker for breast carcinoma and late stage bladder carcinoma. It is positive in a variety of adenocarcinomas and hepatocellular carcinoma, and is generally negative in squamous cell carcinomas.
References: Gastroenterol 2005 129(3):885; Hepatology 2007 46(5):1639; Am J Clin Path 123(1):66; Clin Biochem 35(4):327; Histopath 1993 23(1):45
Target
Human KRT18 / CK18 / Cytokeratin 18
Synonyms
KRT18 | CK-18 | Cytokeratin 18 | Cytokeratin-18 | K18 | Keratin 18 | CYK18 | Keratin-18
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Recombinant Monoclonal
Clone
KRT18/2808R
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified.
Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human KRT18 protein. Antigen Molecular Weight: 45 kDa
Specificity
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Applications
- IHC - Paraffin (1:100)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (1 - 2 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot (0.5-2 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 min at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KRT18 / CK18 / Cytokeratin 18
Validation
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ KRT18 / CK18 / Cytokeratin 18 Antibody (clone KRT18/2808R) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B16286 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mammal
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
