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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-B6047-50 50 µl (1 mg/ml) $425 
HSPA9 / Mortalin / GRP75 Antibody - Anti-GRP75 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
HSPA9 / Mortalin / GRP75 Antibody - Immunofluorescence analysis of COS7 cells, using GRP75 Antibody. The picture on the right is blocked with the synthesized peptide.
HSPA9 / Mortalin / GRP75 Antibody - Western blot analysis of lysates from COS7 cells, using GRP75 Antibody. The lane on the right is blocked with the synthesized peptide.
HSPA9 / Mortalin / GRP75 Antibody - Anti-GRP75 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
HSPA9 / Mortalin / GRP75 Antibody - Immunofluorescence analysis of COS7 cells, using GRP75 Antibody. The picture on the right is blocked with the synthesized peptide.
HSPA9 / Mortalin / GRP75 Antibody - Western blot analysis of lysates from COS7 cells, using GRP75 Antibody. The lane on the right is blocked with the synthesized peptide.
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PathPlus™ Polyclonal Rabbit anti‑Human HSPA9 / Mortalin / GRP75 Antibody (aa630‑679, IHC, IF, WB) LS‑B6047

PathPlus™ Polyclonal Rabbit anti‑Human HSPA9 / Mortalin / GRP75 Antibody (aa630‑679, IHC, IF, WB) LS‑B6047

Note: This antibody replaces LS-C118472
Antibody:
HSPA9 / Mortalin / GRP75 Rabbit anti-Human Polyclonal (aa630-679) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B6047-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HSPA9 / Mortalin / GRP75 Rabbit anti-Human Polyclonal (aa630-679) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HSPA9 (Mortalin, GRP75) is a heat shock protein involved in cell proliferation, stress response and mitochondria maintenance. HSPA9 is of interest in neurodegenerative diseases including Alzheimer’s and Parkinson’s disease; it may have protective chaperone functions in the brain that help degrade and maintain lowered levels of aggregated or improperly folded proteins. In immunohistochemistry, HSPA9 has granular cytoplasmic positivity in all tissues. References: Front Endocrinol (Lausanne). 2018; 9: 196, PMID: 29755410; J Alzheimers Dis. 2014;38(1):165-70. doi: 10.3233/JAD-130428, PMID: 23948933; J Alzheimers Dis. 2014;38(1):165-70, PMID: 23948933
Target
Human HSPA9 / Mortalin / GRP75
Synonyms
HSPA9 | Heat shock 70 kDa protein 9 | GRP-75 | Heat shock 70kD protein 9B | GRP75 | Mortalin-2 | Mortalin, perinuclear | Mot-2 | MOT2 | Peptide-binding protein 74 | MOT | MTHSP75 | p66-mortalin | PBP74 | CSA | HSPA9B | Mortalin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human GRP75. (AA Range: 630-679)
Epitope
aa630-679
Specificity
GRP75 Antibody detects endogenous levels of total GRP75 protein.
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot: Predicted MW 73 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA9 / Mortalin / GRP75
P38646 NM_004134 NP_004125.3

Validation

HSPA9 / Mortalin / GRP75 Antibody - Anti-GRP75 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-GRP75 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-GRP75 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

LSBio Ratings

PathPlus™ HSPA9 / Mortalin / GRP75 Antibody (aa630-679) for IHC, IF/Immunofluorescence, WB/Western LS-B6047 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/5/2020