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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B11166-50 50 µg (0.5 mg/ml) $425 
HDAC6 Antibody - Anti-HDAC6 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HDAC6 Antibody - HDAC6 antibody (1 ug/ml) staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HDAC6 Antibody - Anti-HDAC6 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HDAC6 Antibody - HDAC6 antibody (1 ug/ml) staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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PathPlus™ HDAC6 Antibody aa1100‑C‑term Polyclonal IHC,Peptide‑ELISA,WB LS‑B11166

PathPlus™ HDAC6 Antibody aa1100‑C‑term Polyclonal IHC,Peptide‑ELISA,WB LS‑B11166

Note: This antibody replaces LS-C205019
Antibody:
Goat Polyclonal to Human HDAC6
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B11166-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human HDAC6
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC6 is a histone deacetylase that represses transcription and is involved in cell cycle progression, development, and regulation of gene expression. HDAC6 also plays an important role in cell migration in normal tissue and in cancer via its regulation of tubulin acetylation and depolymerization. Variable levels of HDAC6 dysregulation are associated with survival in different cancer types. For example, HDAC6 overexpression is associated positively with survival in ER-positive breast cancer, cutaneous T-Cell lymphoma and diffuse large B-Cell lymphoma, and with poor prognosis in peripheral T-Cell lymphoma and ER-negative breast cancer. Downregulation of HDAC6 is associated with poor survival in chronic lymphocytic leukemia. In immunohistochemistry of normal tissue, HDAC6 has broad cytoplasmic expression throughout the body, with strongest positivity in seminiferous duct cells and renal tubules. J Cancer Res Ther. 2014 Jul-Sep;10(3):469-78, PMID: 25313724; Cold Spring Harb Perspect Med. 2016 Oct; 6(10): a026831, PMID: 27599530; Oncogene. 2007 Aug. 26: 5420–5432, PMID: 17694083; J Cell Sci. 2007 Apr 15;120(Pt 8):1469-79, PMID: 17389687; J Breast Cancer. 2014 Dec; 17(4): 323–331, PMID: 25548579, PMID: 29180246
Target
Human HDAC6
Host
Goat
Reactivity
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A peptide at the C terminal residues of human HDAC6 protein
Blocking Peptide
LS-E46588 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11166. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1100-C-term
Specificity
Human HDAC6.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 150kD band observed in nuclear lysates of cell lines NIH3T3, and HeLa (calculated MW of 131kD according to NP_006035.2). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC6
Q9UBN7 NM_006044 NP_006035.2

Validation

HDAC6 Antibody - Anti-HDAC6 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HDAC6 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HDAC6 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 11/12/2019