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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B215-0.1 0.1 mg (1 mg/ml) $425 
EP300 / p300 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
EP300 / p300 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
EP300 / p300 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
EP300 / p300 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
EP300 / p300 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
EP300 / p300 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PathPlus™ Monoclonal EP300 / p300 Antibody (clone RW128, aa1572‑2371, IHC, IF, WB) LS‑B215

PathPlus™ Monoclonal EP300 / p300 Antibody (clone RW128, aa1572‑2371, IHC, IF, WB) LS‑B215

Note: This antibody replaces LS-C2747, LS-C91230, LS-C91229, LS-C2745
Antibody:
Mouse Monoclonal to Human EP300 / p300
Application:
IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Mustelid, Primate
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B215-0.1
0.1 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human EP300 / p300
Application:
IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Mustelid, Primate
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
EP300 is a histone acetyltransferase that regulates transcription via chromatin remodeling. It acetylates all four core histones in nucleosomes. EP300 mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. It also plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. EP300 can also promote cardiac myocyte enlargement. In infectious disease, it binds to and may be involved in the transforming capacity of the adenovirus E1A protein, and in case of HIV-1 infection, it is recruited by the viral protein Tat. It regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. In immunohistochemistry, EP300 has nuclear and cytoplasmic positivity in a majority of tissues throughout the body. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804;
Target
Human EP300 / p300
Synonyms
EP300 | E1A binding protein p300 | Histone acetyltransferase p300 | KAT3B | p300 HAT | E1A-associated protein p300 | E1A-binding protein, 300kD | p300 | RSTS2
Host
Mouse
Reactivity
Human, Mouse, Rat, Mustelid, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
RW128
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550, Biotin, DY650, DY488, DY550.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:250 - 1:500)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein containing residues 1572-2371 of human p300.
Epitope
aa1572-2371
Specificity
P300 protein. This recognizes residues 1868-1921.
Usage
Immunohistochemistry: LS-B215 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B215 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EP300 / p300
Q09472 NM_001429 NP_001420.2

Validation

EP300 / p300 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

EP300 / p300 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

EP300 / p300 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ EP300 / p300 Antibody (aa1572-2371, clone RW128) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B215 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 3/30/2020