Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B16274-100 100 µg (200 µg/ml) $485 
CHGA / Chromogranin A Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
CHGA / Chromogranin A Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
CHGA / Chromogranin A Antibody - SDS-PAGE Analysis Purified Chromogranin A Rabbit Recombinant Monoclonal Antibody (CHGA/1815R). Confirmation of Purity and Integrity of Antibody.
CHGA / Chromogranin A Antibody - Analysis of Protein Array containing more than 19,000 full-length human proteins using Chromogranin A Rabbit Recombinant Monoclonal Antibody (CHGA/1815R).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
CHGA / Chromogranin A Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
CHGA / Chromogranin A Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
CHGA / Chromogranin A Antibody - SDS-PAGE Analysis Purified Chromogranin A Rabbit Recombinant Monoclonal Antibody (CHGA/1815R). Confirmation of Purity and Integrity of Antibody.
CHGA / Chromogranin A Antibody - Analysis of Protein Array containing more than 19,000 full-length human proteins using Chromogranin A Rabbit Recombinant Monoclonal Antibody (CHGA/1815R).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
1 of 4
2 of 4
3 of 4
4 of 4

PathPlus™ Recombinant Monoclonal Rabbit anti‑Human CHGA / Chromogranin A Antibody (clone CHGA/1815R, IHC) LS‑B16274

PathPlus™ Recombinant Monoclonal Rabbit anti‑Human CHGA / Chromogranin A Antibody (clone CHGA/1815R, IHC) LS‑B16274

Note: This antibody replaces LS-C751102
Antibody:
CHGA / Chromogranin A Rabbit anti-Human Recombinant Monoclonal (CHGA/1815R) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B16274-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CHGA / Chromogranin A Rabbit anti-Human Recombinant Monoclonal (CHGA/1815R) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CHGA (Chromogranin A) encodes a protein involved in the production of secretory vesicles in endocrine and neuroendocrine cells. Levels of this protein correlate with the number of secretory vesicles present, and staining of this protein is useful for subtyping neuroendocrine tumors in multiple tissues including the lung. Serum of patients with small cell lung cancer can be checked for circulating CHGA, and in this context it has been shown to have more sensitivity than neuroendocrine marker NSE. Sensitivity of CHGA is expected to increase with stage. Staining for Chromogranin A is expected to be cytoplasmic. References: Gut, 2016; Syversen, 2004; Taneja, 2004; Seregni, 2001; Endoc Rev 2011 32:755
Target
Human CHGA / Chromogranin A
Synonyms
CHGA | Chromogranin A | Chromogranin-A | Pancreastatin | Pituitary secretory protein I | Vasostatin I | SP-I | Vasostatin II
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Rat
Clonality
IgG1,k Recombinant Monoclonal
Clone
CHGA/1815R
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human Chromogranin A (CHGA) protein. Antigen Molecular Weight: 68-75 kDa
Specificity
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Applications
  • IHC - Paraffin (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHGA / Chromogranin A
P10645 NM_001275 NP_001266.1

Validation

CHGA / Chromogranin A Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

CHGA / Chromogranin A Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ CHGA / Chromogranin A Antibody (clone CHGA/1815R) for IHC LS-B16274 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/17/2021