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Catalog Number Size Price
LS-B8765-50 50 µg (1 mg/ml) $485 
CD3 Antibody - Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ Monoclonal Rat anti‑Human CD3 Antibody (clone CD3‑12, IHC, WB) LS‑B8765

PathPlus™ Monoclonal Rat anti‑Human CD3 Antibody (clone CD3‑12, IHC, WB) LS‑B8765

Note: This antibody replaces LS-C121496
Antibody:
CD3 Rat anti-Human Monoclonal (CD3-12) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B8765-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CD3 Rat anti-Human Monoclonal (CD3-12) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD3 is an integral membrane glycoprotein that associates with T-cell antigen receptor (TCR) and is required for TCR cell surface expression and signal transduction. It is a highly specific marker for normal T-lymphocytes, thymocytes, and T-cell malignancies. It is negative in B-cells. References: Chetty and Gatter, 1994; Smith-Garvin et al. 2009
Target
Human CD3
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
CD3-12
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Synthetic peptide sequence derived from cytoplasmic epitope of CD3.
Specificity
Recognizes a highly conserved epitope of the CD3 molecule expressed by T lymphocytes.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:1000)
  • Western blot (1:50 - 1:100)
  • Flow Cytometry (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Western Blot. Flow Cytometry: 1:50-1:100; 10 ul labels 10^6 cells in 100ul. Immunohistochemistry (frozen): 1:1000.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

CD3 Antibody - Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ CD3 Antibody (clone CD3-12) for IHC, WB/Western, Flow LS-B8765 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/22/2021