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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B6274-50 50 µl (1 mg/ml) $425 
CCNE1 / Cyclin E1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CCNE1 / Cyclin E1 Antibody - Anti-CCNE1 / Cyclin E1 antibody IHC of human-lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
CCNE1 / Cyclin E1 Antibody - Immunofluorescence analysis of A549 cells, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.
CCNE1 / Cyclin E1 Antibody - Western blot analysis of lysates from K562 cells, using Cyclin E1 Antibody. The lane on the right is blocked with the synthesized peptide.
CCNE1 / Cyclin E1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CCNE1 / Cyclin E1 Antibody - Anti-CCNE1 / Cyclin E1 antibody IHC of human-lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
CCNE1 / Cyclin E1 Antibody - Immunofluorescence analysis of A549 cells, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.
CCNE1 / Cyclin E1 Antibody - Western blot analysis of lysates from K562 cells, using Cyclin E1 Antibody. The lane on the right is blocked with the synthesized peptide.
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PathPlus™ Polyclonal Rabbit anti‑Human CCNE1 / Cyclin E1 Antibody (aa91‑140, IHC, IF, WB) LS‑B6274

PathPlus™ Polyclonal Rabbit anti‑Human CCNE1 / Cyclin E1 Antibody (aa91‑140, IHC, IF, WB) LS‑B6274

Note: This antibody replaces LS-C118425
Antibody:
CCNE1 / Cyclin E1 Rabbit anti-Human Polyclonal (aa91-140) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B6274-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CCNE1 / Cyclin E1 Rabbit anti-Human Polyclonal (aa91-140) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CCNE1 (Cyclin E1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. It forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein is involved in the phosphorylation of NPAT protein, which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In immunohistochemistry, CCNE1 has variable levels of nuclear and cytoplasmic positivity in most tissues throughout the body. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804;
Target
Human CCNE1 / Cyclin E1
Synonyms
CCNE1 | Cyclin E1 | CCNE | Cyclin Es | Cyclin Et | G1/S-specific cyclin-E1 | G1/s-specific cyclin e | Cyclin E
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human Cyclin E1. (AA Range: 91-140)
Epitope
aa91-140
Specificity
Cyclin E1 Antibody detects endogenous levels of total Cyclin E1 protein.
Applications
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot: Predicted MW 47 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCNE1 / Cyclin E1
P24864 NM_001238 NP_001229.1

Validation

CCNE1 / Cyclin E1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

CCNE1 / Cyclin E1 Antibody - Anti-CCNE1 / Cyclin E1 antibody IHC of human-lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CCNE1 / Cyclin E1 antibody IHC of human-lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CCNE1 / Cyclin E1 antibody IHC of human-lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

LSBio Ratings

PathPlus™ CCNE1 / Cyclin E1 Antibody (aa91-140) for IHC, IF/Immunofluorescence, WB/Western LS-B6274 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/5/2020