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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B6276-50 50 µl (0.156 mg/ml) $425 
Actin Antibody - Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Actin Antibody - Immunofluorescent staining of HeLa cells using anti-Actin antibody.
Actin Antibody - Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.
Actin Antibody - Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Actin Antibody - Immunofluorescent staining of HeLa cells using anti-Actin antibody.
Actin Antibody - Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.
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PathPlus™ Monoclonal Rabbit anti‑Human Actin Antibody (clone EP184E, N‑Terminus, IHC, WB) LS‑B6276

PathPlus™ Monoclonal Rabbit anti‑Human Actin Antibody (clone EP184E, N‑Terminus, IHC, WB) LS‑B6276

Note: This antibody replaces LS-C49807
Antibody:
Actin Rabbit anti-Human Monoclonal (N-Terminus) (EP184E) Antibody
Application:
IHC-P, ICC, WB, IP, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B6276-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Actin Rabbit anti-Human Monoclonal (N-Terminus) (EP184E) Antibody
Application:
IHC-P, ICC, WB, IP, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Actins are ubiquitously expressed proteins that are a major component of the cytoskeleton. Actin is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues. It regulates motility and controls cells structure in both muscle and non-muscle cells (where it is present in the cytoplasm as well). Pan-actin antibodies can be used to identify actin expression and localization in normal and disease states. Pan-actin antibodies can be used to label myotubes, myoblasts and fibroblasts. They are useful for determining total actin levels to compare against the differential expression of specific actins in normal versus diseased tissue, such as kidney tissue in diabetes. References: Cytoskeleton (Hoboken). 2010 Nov;67(11):729-43, PMID: 20862689; Clin Proteom 8, 15 (2011) doi:10.1186/1559-0275-8-15; Molecular & Cellular Proteomics. 2012. 11 (8): 255-271, DOI: 10.1074/mcp.M111.015099
Target
Human Actin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
EP184E
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to residues near the N-Terminus of human Actin.
Epitope
N-Terminus
Applications
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:250 - 1:500)
  • Western blot (1:1000)
  • Immunoprecipitation (1:20)
  • Flow Cytometry (1:20)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Tris-Glycine-NaCl or PBS, pH 7.2-7.4, 40-50% Glycerol, 0.01% sodium azide, 0.05% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Actin Antibody - Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

PathPlus™ Actin Antibody (N-Terminus, clone EP184E) for IHC, ICC, WB/Western, IP, Flow LS-B6276 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/26/2020