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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B7484-50 50 µg (1 mg/ml) $425 
SERPINB5 / Maspin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
SERPINB5 / Maspin Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
SERPINB5 / Maspin Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
SERPINB5 / Maspin Antibody - Western Blot (reducing) of Maspin antibody
SERPINB5 / Maspin Antibody - Sandwich ELISA of Maspin antibody
SERPINB5 / Maspin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
SERPINB5 / Maspin Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
SERPINB5 / Maspin Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
SERPINB5 / Maspin Antibody - Western Blot (reducing) of Maspin antibody
SERPINB5 / Maspin Antibody - Sandwich ELISA of Maspin antibody
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PathPlus™ Polyclonal Rabbit anti‑Human SERPINB5 / Maspin Antibody (IHC, WB) LS‑B7484

PathPlus™ Polyclonal Rabbit anti‑Human SERPINB5 / Maspin Antibody (IHC, WB) LS‑B7484

Note: This antibody replaces LS-C104541
Antibody:
Rabbit Polyclonal to Human SERPINB5 / Maspin
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B7484-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human SERPINB5 / Maspin
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
SERPINB5 (maspin) is a serine protease inhibitor regulated by TP53 that has putative tumor suppressor functions. It is involved in mammary gland development via EGFR signaling. In cancer, both the degree of expression and the cellular localization of SERPINB5 correlates with stage and progression in some cancers. For example, SERPINB5 has generally nuclear positivity in normal epithelium, and primarily cytoplasmic localization of SERPINB5 is associated with tumorigenesis in lung, prostate and esophageal adenocarcinoma. Cytoplasmic SERPINB5 may be a useful early diagnostic biomarker for the transformation of normal epithelia and Barrett’s esophagus into esophageal adenocarcinoma; overall expression of SERPINB5 appears to decrease with stage and cellular distribution trends from nuclear to cytoplasmic alongside progression. SERPINB5 expression is lost in invasive subtypes, and predominantly cytoplasmic expression may be indicative of pre-neoplastic lesions with a greater risk of progression to adenocarcinoma. Dzinic et al have indicated that SERPINB5 may only have tumor suppressive functions while localized to the nucleus, where it suppresses HDAC1 suppression, plays a role in chromatin folding and contributes to genetic stability. Predominantly cytoplasmic expression and absence in the nucleus may thus lead to an increase in DNA mutations and ultimately promote tumor cell survival. In immunohistochemistry of normal tissue, SERPINB5 has both cytoplasmic and nuclear positivity in the esophagus, prostate, skin, intestine, lung, cerebral cortex, thymus, testis and tongue. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Neurol Res. 2010 Apr;32(3):303-8, PMID: 19909580; Science. 263 (5146): 526–9, PMID: 8290962; PLoS One. 2016; 11(7): e0159856, PMID: 27447178; PLoS One. 2019 Apr 19;14(4):e0215089, PMID: 31002675; Cancer Res. 2006 Sep 15;66(18):9323-9, PMID: 16982778
Target
Human SERPINB5 / Maspin
Synonyms
SERPINB5 | Maspin | Peptidase inhibitor 5 | PI5 | Protease inhibitor 5 (maspin) | Serpin B5 | PI-5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC - Paraffin (2.5 - 5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E.coli derived recombinant Human Maspin.
Specificity
Human Maspin
Usage
Immunohistochemistry: LS-B7484 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 ug/ml is recommended. ELISA: To detect hMaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (LS-C104540) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMaspin. Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINB5 / Maspin
P36952 NM_002639 NP_002630.2

Validation

SERPINB5 / Maspin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

SERPINB5 / Maspin Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

SERPINB5 / Maspin Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ SERPINB5 / Maspin Antibody for IHC, WB/Western, ELISA LS-B7484 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 7/12/2020