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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-A8392-50 50 µg (1 mg/ml) $425 
PRKCZ / PKC-Zeta Antibody - Brain, Hypothalamus
PRKCZ / PKC-Zeta Antibody - Brain, Alzheimer's disease senile plaque
PRKCZ / PKC-Zeta Antibody - Anti-PRKCZ / PKC-Zeta antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PRKCZ / PKC-Zeta Antibody - Brain, Hypothalamus
PRKCZ / PKC-Zeta Antibody - Brain, Alzheimer's disease senile plaque
PRKCZ / PKC-Zeta Antibody - Anti-PRKCZ / PKC-Zeta antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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PathPlus™ Polyclonal PRKCZ / PKC‑Zeta Antibody (N‑Terminus, IHC) LS‑A8392

PathPlus™ Polyclonal PRKCZ / PKC‑Zeta Antibody (N‑Terminus, IHC) LS‑A8392

Note: This antibody replaces LS-C91499, LS-C42431, LS-C122221
Antibody:
Rabbit Polyclonal to Human PRKCZ / PKC-Zeta
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A8392-50
50 µg
1 mg/ml

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human PRKCZ / PKC-Zeta
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Protein kinase C, zeta (PRKCZ) is a kinase required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment, it functions as a downstream effector of PI3K and contributes to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. PRKCZ participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, it forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In the inflammatory response, it is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. Furthermore, in association with FEZ1, it stimulates neuronal differentiation in PC12 cells. In immunohistochemistry, it has membranous and cytoplasmic positivity throughout the body. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human PRKCZ / PKC-Zeta
Synonyms
PRKCZ | PKC-ZETA | PKC2 | Pkcz | PKCzeta | Protein kinase c zeta | Protein kinase C zeta type | NPKC-zeta | PKC Zeta | Protein kinase C, zeta | Protein kinase Czeta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 19 amino acid peptide near N-Terminus of human PRKCZ / PKC-Zeta. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (95%); Marmoset, Mouse, Rat, Rabbit (89%).
Blocking Peptide
LS-E29402 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A8392. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human PRKCZ / PKC-Zeta. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5-3 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCZ / PKC-Zeta
Q05513 NM_002744 NP_002735.3

Validation

PRKCZ / PKC-Zeta Antibody - Brain, Hypothalamus
Brain, Hypothalamus
Brain, Hypothalamus

PRKCZ / PKC-Zeta Antibody - Brain, Alzheimer's disease senile plaque
Brain, Alzheimer's disease senile plaque
Brain, Alzheimer's disease senile plaque

PRKCZ / PKC-Zeta Antibody - Anti-PRKCZ / PKC-Zeta antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PRKCZ / PKC-Zeta antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PRKCZ / PKC-Zeta antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ PRKCZ / PKC-Zeta Antibody (N-Terminus) for IHC, ELISA LS-A8392 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 3/31/2020