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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B1753-50 50 µg (0.5 mg/ml) $425 
NET1 / ARHGEF8 Antibody - Anti-NET1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PathPlus™ Polyclonal Goat anti‑Human NET1 / ARHGEF8 Antibody (aa396‑409, IHC) LS‑B1753

PathPlus™ Polyclonal Goat anti‑Human NET1 / ARHGEF8 Antibody (aa396‑409, IHC) LS‑B1753

Note: This antibody replaces LS-C47574, LS-C73334
Antibody:
Goat Polyclonal to Human NET1 / ARHGEF8
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1753-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human NET1 / ARHGEF8
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
NET1 (Neuroepithelial cell-transforming gene 1, Net1A, ARHGEF8) is a guanine nucleotide exchange factor protein that activates RhoA GTPase. Studies in zebrafish have illustrated that NET1 is important for embryogenesis and in establishing the body plan. Furthermore, variants in NET1 are associated with oppositional defiant disorder (ODD) and attention-deficit/hyperactivity disorder (ADHD) in humans. In cancer, deletion mutations in NET1 cause the protein to be re-localized to the cytosol, where it aberrantly activates RhoA and induces transformation. In immunohistochemistry, NET1 has cytoplasmic positivity in the brain, gastrointestinal tract, muscle, reproductive tissues, kidney, liver and some immune tissues. References: J Child Psychol Psychiatry. 2015 Jan;56(1):58-66, PMID: 24942521; Zhonghua Yi Xue Za Zhi. 2015 Nov 3;95(41):3351-6, PMID: 26812975; Cell Res. 2017 Feb; 27(2): 202–225, PMID: 27910850; Mol Cell Biol. 2007 Dec; 27(24): 8683–8697, PMID: 17938206; Journal of Cell Science 2018 131: jcs204644, DOI: 10.1242/jcs.204644
Target
Human NET1 / ARHGEF8
Synonyms
NET1 | ARHGEF8 | NET1A | Proto-oncogene p65 Net1
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2NP_001040625.1.
Blocking Peptide
LS-E26108 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1753. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa396-409
Specificity
Human NET1. This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Usage
Immunohistochemistry: LS-B1753 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1753 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NET1 / ARHGEF8
Q7Z628 NM_005863 NP_005854.2

Validation

NET1 / ARHGEF8 Antibody - Anti-NET1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NET1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NET1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

PathPlus™ NET1 / ARHGEF8 Antibody (aa396-409) for IHC LS-B1753 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 7/3/2020