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Catalog Number Size Price
LS-B7181-50 50 µg (1 mg/ml) $425 
NES / Nestin Antibody - Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
NES / Nestin Antibody - Immunostaining of intracellular nestin filaments in U251 cells
NES / Nestin Antibody - Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using NES / Nestin (1:100 dilution).
NES / Nestin Antibody - Immunoblotting of U251-cell lysate showing reactivity with nestin
NES / Nestin Antibody - Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
NES / Nestin Antibody - Immunostaining of intracellular nestin filaments in U251 cells
NES / Nestin Antibody - Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using NES / Nestin (1:100 dilution).
NES / Nestin Antibody - Immunoblotting of U251-cell lysate showing reactivity with nestin
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PathPlus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 10C2, IHC, WB) LS‑B7181

PathPlus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 10C2, IHC, WB) LS‑B7181

Note: This antibody replaces LS-C146775
Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7181-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nestin is an intermediate filament protein involved in regulating filament assembly, cell remodeling, regeneration, and neural development during embryogenesis. It is a marker of cell migration and proliferation and of progenitor neural cells. Nestin is mostly expressed in cells during development and expression is lost in terminally differentiated cells. It is also expressed in regenerating tissues and as part of the stress response to injury. It stains positively in progenitor neuroepithelial stem cells in the central nervous system (CNS) and in precursor cells of the peripheral nervous system (PNS), and in a subset of adult neurons in the basal forebrain. Nestin also sees expression outside of the CNS and PNS, generally in cells that are pluripotent or undifferentiated, highly mobile, or responding to injury or disease. It may show positivity in endothelial cells, myoepithelial cells, renal glomeruli, cardiac myocytes, the retina, striated muscle, the pancreas, the liver, kidneys, skin, teeth, testes, and the adrenal gland. In cancer, it is expressed in various tumors of the central nervous system, including astrocytic gliomas, neurocytomas, neuroblastomas, ependymomas, medulloblastomas and Schwannomas. References: Journal of Clinical Pathology 2005;58:222-223; PLoS ONE. 2010 Dec. 6(4): e18535, DOI:10.1371/journal.pone.0018535; J Interdiscipl Histopathol. 2016. 4, DOI: 10.5455/jihp.20160420021353; Histol Histopathol. 2005 Apr;20(2):665-71; Neuroscience and Behavioral Physiology. 2008 Feb. 38(2):165–169, DOI: 10.1007/s11055-008-0025-z;
Target
Human NES / Nestin
Synonyms
NES | Nestin | Nbla00170
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1 Monoclonal
Clone
10C2
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:100 - 1:200)
  • ICC
  • Western blot (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 150 AA fragment from the cloned human nestin was conjugated to glutathione S-transferase
Specificity
Reacts exclusively with the human nestin protein. No cross-reactivity to rodent nestin.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

Validation

NES / Nestin Antibody - Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

PathPlus™ NES / Nestin Antibody (clone 10C2) for IHC, ICC, WB/Western LS-B7181 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 5/30/2020