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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-A7175-50 50 µg (1 mg/ml) $425 
NEK9 Antibody - Non-Hodgkin's lymphoma
NEK9 Antibody - Prostate carcinoma
NEK9 Antibody - Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NEK9 Antibody - Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NEK9 Antibody - Prostate, Carcinoma
NEK9 Antibody - Non-Hodgkin's lymphoma
NEK9 Antibody - Prostate carcinoma
NEK9 Antibody - Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NEK9 Antibody - Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NEK9 Antibody - Prostate, Carcinoma
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PathPlus™ Polyclonal NEK9 Antibody (Kinase Domain, IHC) LS‑A7175

PathPlus™ Polyclonal NEK9 Antibody (Kinase Domain, IHC) LS‑A7175

Antibody:
Rabbit Polyclonal to Human NEK9
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Bat, Bovine, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A7175-50
50 µg
1 mg/ml

Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NEK9
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Bat, Bovine, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human NEK9
Synonyms
NEK9 | KIAA1995 | NERCC | NERCC1 | Nercc1 kinase | NIMA-related kinase 8 | NIMA-related kinase Nek8 | NIMA-related kinase 9 | NimA-related protein kinase 9
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bat, Bovine, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Rat, Dog, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 20 amino acid peptide from kinase domain of human NEK9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard (100%); Rat, Dog (95%); Xenopus (90%); Pufferfish (80%).
Blocking Peptide
LS-E29235 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A7175. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Kinase Domain
Specificity
Human NEK9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEK9
Q8TD19 NM_033116 NP_149107.4

Validation

NEK9 Antibody - Non-Hodgkin's lymphoma
Non-Hodgkin's lymphoma
Non-Hodgkin's lymphoma

NEK9 Antibody - Prostate carcinoma
Prostate carcinoma
Prostate carcinoma

NEK9 Antibody - Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

NEK9 Antibody - Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

NEK9 Antibody - Prostate, Carcinoma
Prostate, Carcinoma
Prostate, Carcinoma

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Requested From: United States
Date Requested: 2/21/2020