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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-A1591-50 50 µg (1 mg/ml) $425 
GPR75 Antibody - Brain, Cerebellum, Purkinje neuron
GPR75 Antibody - Skin, Melanoma
GPR75 Antibody - Anti-GPR75 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
GPR75 Antibody - Anti-GPR75 antibody IHC of human brain, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
GPR75 Antibody - Brain, Cerebellum, Purkinje neuron
GPR75 Antibody - Skin, Melanoma
GPR75 Antibody - Anti-GPR75 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
GPR75 Antibody - Anti-GPR75 antibody IHC of human brain, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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PathPlus™ GPR75 Antibody Cytoplasmic Domain Polyclonal IHC LS‑A1591

PathPlus™ GPR75 Antibody Cytoplasmic Domain Polyclonal IHC LS‑A1591

Antibody:
Rabbit Polyclonal to Human GPR75
Application:
IHC, IHC-P
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A1591-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPR75
Application:
IHC, IHC-P
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GPR75 is an orphan GPCR that has been identified as a putative receptor for the eicosanoid 20-HETE. It is a critical component of 20-HETE’s signaling and bioactions in vitro and in vivo, regulating vascular tone and function. Circ Res. 2017 May 26; 120(11): 1776–1788.
Target
Human GPR75
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR75. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Bovine, Pig (95%); Bat, Elephant, Panda, Rabbit, Horse (89%); Mouse, Rat, Xenopus (84%).
Blocking Peptide
LS-E28344 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1591. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human GPR75. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR75
O95800 NM_006794 NP_006785.1

Validation

GPR75 Antibody - Brain, Cerebellum, Purkinje neuron
Brain, Cerebellum, Purkinje neuron
Brain, Cerebellum, Purkinje neuron

GPR75 Antibody - Skin, Melanoma
Skin, Melanoma
Skin, Melanoma

GPR75 Antibody - Anti-GPR75 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

GPR75 Antibody - Anti-GPR75 antibody IHC of human brain, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human brain, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human brain, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 12/16/2019