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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B4335-50 50 µg (0.48 mg/ml) $495 
GPR3 Antibody - Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GPR3 Antibody - Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GPR3 Antibody - GPR3 monoclonal antibody, clone 3B4-G3 Western blot of GPR3 expression in Jurkat.
GPR3 Antibody - Western blot of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody clone 3B4-G3.
GPR3 Antibody - Detection limit for recombinant GST tagged GPR3 is approximately 0.03 ng/ml as a capture antibody.
GPR3 Antibody - Western blot of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody, clone 3B4-G3. GAPDH ( 36.1 kD ) used as specificity a.
GPR3 Antibody - Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GPR3 Antibody - Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GPR3 Antibody - GPR3 monoclonal antibody, clone 3B4-G3 Western blot of GPR3 expression in Jurkat.
GPR3 Antibody - Western blot of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody clone 3B4-G3.
GPR3 Antibody - Detection limit for recombinant GST tagged GPR3 is approximately 0.03 ng/ml as a capture antibody.
GPR3 Antibody - Western blot of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody, clone 3B4-G3. GAPDH ( 36.1 kD ) used as specificity a.
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PathPlus™ Monoclonal Mouse anti‑Human GPR3 Antibody (clone 3B4‑G3, IHC, WB) LS‑B4335

PathPlus™ Monoclonal Mouse anti‑Human GPR3 Antibody (clone 3B4‑G3, IHC, WB) LS‑B4335

Note: This antibody replaces LS-C104960
Antibody:
Mouse Monoclonal to Human GPR3
Application:
IHC, IHC-P, WB, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$495 
LS-B4335-50
50 µg
0.48 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human GPR3
Application:
IHC, IHC-P, WB, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
G protein-coupled receptor 3 (GPR3) is a constitutively active lysophospholipid/lysosphingolipid receptor. GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle where GPR3 maintains meiotic arrest in mouse oocytes. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and mice deficient in GPR3 exhibit premature ovarian failure due to early oocyte aging (Ledent et al. 2005). References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human GPR3
Synonyms
GPR3 | ACCA | ACCA orphan receptor | G protein-coupled receptor 3 | GPCR3 | Hacca | G-protein coupled receptor 3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
3B4-G3
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
  • RNA interference
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR3 (AAH32702, 1 aa ~ 331 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control Jurkat. Western Blot (Transfected lysate) - positive control transfected 293T cell line. RNAi Knockdown (Antibody validated).
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR3
P46089 NM_005281 NP_005272.1

Validation

GPR3 Antibody - Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

GPR3 Antibody - Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

PathPlus™ GPR3 Antibody (clone 3B4-G3) for IHC, WB/Western, ELISA LS-B4335 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 7/7/2020