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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-A6603-50 50 µg (1 mg/ml) $425 
CRFR1 / CRHR1 Antibody - Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ CRFR1 / CRHR1 Antibody N‑Terminus Polyclonal IHC LS‑A6603

PathPlus™ CRFR1 / CRHR1 Antibody N‑Terminus Polyclonal IHC LS‑A6603

Antibody:
Rabbit Polyclonal to Human CRFR1 / CRHR1
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A6603-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CRFR1 / CRHR1
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Corticotropin releasing hormone receptor 1 (CRHR1) is a G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). CRHR1 has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. CRHR1 promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels and inhibits the activity of the calcium channel CACNA1H. CRHR1 is required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress and plays a role in the response to anxiogenic stimuli. The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human CRFR1 / CRHR1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse (tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (15 - 20 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 15 amino acid peptide from N-terminal extracellular domain of human CRF1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Panda, Horse (100%); Bat, Bovine, Pig, Opossum (93%); Elephant (87%); Sheep, Lizard (80%).
Blocking Peptide
LS-E29147 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A6603. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CRHR2 (73%).
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRFR1 / CRHR1
P34998 NM_004382 P34998

Validation

CRFR1 / CRHR1 Antibody - Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Publications (1)

Does the corticotropin-releasing hormone system play a role in the pathogenesis of lichen planus?. Cemil BC, Yilmazer D, Atas H, Cengiz FP, Canpolat F, Alper M, Gonul M. Postepy dermatologii i alergologii. 2017 August;34:322-327. (Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/13/2019