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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-A8893-50 50 µg (1 mg/ml) $425 
BACE1 / BACE Antibody - Anti-BACE1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ Polyclonal BACE1 / BACE Antibody (Internal, IHC) LS‑A8893

PathPlus™ Polyclonal BACE1 / BACE Antibody (Internal, IHC) LS‑A8893

Note: This antibody replaces LS-C74516
Antibody:
Rabbit Polyclonal to Human BACE1 / BACE
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A8893-50
50 µg
1 mg/ml

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human BACE1 / BACE
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
BACE-1 (beta-secretase 1) is a peptidase that is responsible for the proteolytic processing of the amyloid precursor protein (APP). It cleaves APP at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Amyloid beta peptides are the primary constituent within amyloid beta plaques (senile plaques), which accumulate in the brains of Alzheimer’s patients. Chemical inhibition of BACE-1 has been shown to reduce beta-amyloid in animal and human studies, making it a target of therapeutic intervention for Alzheimer’s Disease. References: Lancet Neurol. 2014 Mar; 13(3): 319–329; Transl Neurodegener. 2017; 6: 23.
Target
Human BACE1 / BACE
Synonyms
BACE1 | APP beta-secretase | Asp 2 | ASP2 | Aspartyl protease 2 | Aspartylprotease 2 | Beta-secretase | Beta-site APP-cleaving enzyme | BACE | HSPC104 | KIAA1149 | B-Secretase | Beta-secretase 1 | Memapsin-2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 15 amino acid peptide from internal region of human BACE1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig (100%); Platypus (87%); Xenopus, Pufferfish, Zebrafish, Stickleback (80%).
Blocking Peptide
LS-E29508 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A8893. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human BACE1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BACE1 / BACE
P56817 NM_012104 NP_036236.1

Validation

BACE1 / BACE Antibody - Anti-BACE1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BACE1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BACE1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ BACE1 / BACE Antibody (Internal) for IHC LS-A8893 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 3/31/2020