Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-A1513-50 50 µg (1 mg/ml) $425 
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRF5 / GPR116 Antibody - Brain, substantia nigra
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human brain, neuron. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRF5 / GPR116 Antibody - Brain, substantia nigra
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human brain, neuron. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

PathPlus™ Polyclonal ADGRF5 / GPR116 Antibody (N‑Terminus, IHC) LS‑A1513

PathPlus™ Polyclonal ADGRF5 / GPR116 Antibody (N‑Terminus, IHC) LS‑A1513

Antibody:
Rabbit Polyclonal to Human ADGRF5 / GPR116
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A1513-50
50 µg
1 mg/ml

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human ADGRF5 / GPR116
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GPR116 (Adgrf5) is a member of the adhesion class of GPCRs (AdGPCRs), which share extracellular domains containing highly conserved GPCR autoproteolysis–inducing (GAIN) domains. In the lung, GPR116 is localized to the plasma membrane of alveolar type I (AT1) and AT2 cells and its RNA is detected in pulmonary endothelial cells. GPR116 is activated by its extracellular domain to activate GNAQ/GNA11, in turn regulating secretion of pulmonary surfactant. The loss of Gpr116 causes early accumulation of surfactant in the lungs, followed producing an emphysema-like pathology. References: JCI Insight. 2017 Jun 2; 2(11): e93700.; Plos One https://doi.org/10.1371/journal.pone.0137949
Target
Human ADGRF5 / GPR116
Synonyms
ADGRF5 | Ig-Hepta homolog | Ig-hepta | G protein-coupled receptor 116 | GPR116 | KIAA0758 | KPG_001
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2 - 4 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 19 amino acid peptide from N-Terminus extracellular domain of human GPR116. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (95%).
Blocking Peptide
LS-E28298 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1513. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human GPR116. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADGRF5 / GPR116
Q8IZF2 NM_015234 AAN46672.1

Validation

ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

ADGRF5 / GPR116 Antibody - Brain, substantia nigra
Brain, substantia nigra
Brain, substantia nigra

ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

ADGRF5 / GPR116 Antibody - Anti-GPR116 antibody IHC of human brain, neuron. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human brain, neuron. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human brain, neuron. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ ADGRF5 / GPR116 Antibody (N-Terminus) for IHC LS-A1513 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Essential regulation of lung surfactant homeostasis by the orphan G protein-coupled receptor GPR116. Yang MY, Hilton MB, Seaman S, Haines DC, Nagashima K, Burks CM, Tessarollo L, Ivanova PT, Brown HA, Umstead TM, Floros J, Chroneos ZC, St Croix B. Cell reports. 2013 May;3:1457-64.

Customer Reviews (0)


Requested From: United States
Date Requested: 4/5/2020