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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-A2035-50 50 µg (1 mg/ml) $425 
ADGRD1 / GPR133 Antibody - Lung, non small cell carcinoma
ADGRD1 / GPR133 Antibody - Brain, Glioblastoma
ADGRD1 / GPR133 Antibody - Brain, Hypothalamus, PVN
ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human ovary, surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRD1 / GPR133 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
ADGRD1 / GPR133 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
ADGRD1 / GPR133 Antibody - Lung, non small cell carcinoma
ADGRD1 / GPR133 Antibody - Brain, Glioblastoma
ADGRD1 / GPR133 Antibody - Brain, Hypothalamus, PVN
ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human ovary, surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ADGRD1 / GPR133 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
ADGRD1 / GPR133 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PathPlus™ ADGRD1 / GPR133 Antibody Cytoplasmic Domain Polyclonal IHC LS‑A2035

PathPlus™ ADGRD1 / GPR133 Antibody Cytoplasmic Domain Polyclonal IHC LS‑A2035

Antibody:
Rabbit Polyclonal to Human ADGRD1 / GPR133
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A2035-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ADGRD1 / GPR133
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
ADGRD1 is an orphan adhesion G protein-coupled receptor that is transcriptionally upregulated by hypoxia in a Hif1a dependent manner. It is a critical regulator of the response to hypoxia and tumor growth in glioblastoma, and its expression levels are inversely correlated with patient survival. The receptor is widely expressed in human tissues. Oncogenesis. 2016 Oct; 5(10): e263.
Target
Human ADGRD1 / GPR133
Host
Rabbit
Reactivity
Human, Monkey, Dog, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bovine, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 - 20 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR133. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog, Panda, Rabbit, Pig (100%); Gibbon, Bovine, Elephant, Horse (95%); Mouse, Hamster (84%).
Blocking Peptide
LS-E28473 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A2035. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human GPR133. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADGRD1 / GPR133
Q6QNK2 AY278561 AAP35063.1

Validation

ADGRD1 / GPR133 Antibody - Lung, non small cell carcinoma
Lung, non small cell carcinoma
Lung, non small cell carcinoma

ADGRD1 / GPR133 Antibody - Brain, Glioblastoma
Brain, Glioblastoma
Brain, Glioblastoma

ADGRD1 / GPR133 Antibody - Brain, Hypothalamus, PVN
Brain, Hypothalamus, PVN
Brain, Hypothalamus, PVN

ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR133 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

ADGRD1 / GPR133 Antibody - Anti-GPR133 antibody IHC of human ovary, surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR133 antibody IHC of human ovary, surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR133 antibody IHC of human ovary, surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

ADGRD1 / GPR133 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

ADGRD1 / GPR133 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Requested From: United States
Date Requested: 11/13/2019