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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-A1236-50 50 µg (1 mg/ml) $425 
GPR49 / LGR5 Antibody - Brain, Caudate
GPR49 / LGR5 Antibody - Brain, glioblastoma
GPR49 / LGR5 Antibody - Brain, hippocampus
GPR49 / LGR5 Antibody - Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Brain, Caudate
GPR49 / LGR5 Antibody - Brain, glioblastoma
GPR49 / LGR5 Antibody - Brain, hippocampus
GPR49 / LGR5 Antibody - Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
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PathPlus™ GPR49 / LGR5 Antibody Cytoplasmic Domain Polyclonal IF,IHC LS‑A1236

PathPlus™ GPR49 / LGR5 Antibody Cytoplasmic Domain Polyclonal IF,IHC LS‑A1236

Antibody:
Rabbit Polyclonal to Human GPR49 / LGR5
Application:
IHC, IHC-P, IF
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A1236-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPR49 / LGR5
Application:
IHC, IHC-P, IF
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Leucine-rich repeat containing G protein-coupled receptor 5 (LGR5, GPR49, FEX) is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and in hair follicles. Upon binding to R-spondins, such as RSPO1, RSPO2, RSPO3 or RSPO4, it interacts with phosphorylated LRP6 and frizzled receptors and triggers the canonical Wnt signaling pathway to increase expression of target genes. It is involved in the development and maintenance of adult intestinal stem cells during postembryonic development. In immunohistochemistry, it is often used as a marker for LGR5+ multipotent stem cells. It has membranous positivity in various tissues including muscle, the spinal cord, the brain, crypt base columnar cells in the small intestine and in the colon, nascent nephron cell clusters in the developing kidney, populations of cells in the stomach, and the placenta. It is also a useful marker for characterizing stem cells, stem-ness and also poor prognosis in premalignant and cancerous tissues such as glioblastoma, cervical cancer and colorectal carcinoma. The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Gastroenterology. 2010. 138 (5): 1681–96, PMID: 20417836; ScientificWorldJournal. 2008 Nov 23;8:1168-76, PMID: 19030762; Cell Death Dis. 2017 Sep 7;8(9):e3039, PMID: 28880275; Brain Pathol. 2013 Jan;23(1):60-72, PMID: 22805276;
Target
Human GPR49 / LGR5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Hamster, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 20 amino acid peptide from 1st cytoplasmic domain of human GPR49 / LGR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Marmoset, Bovine, Bat (95%); Hamster, Elephant, Panda, Rabbit (90%); Mouse, Rat, Opossum (85%).
Blocking Peptide
LS-E28194 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1236. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human GPR49 / LGR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.09% Sodium Azide, 10% Glycerol
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR49 / LGR5
O75473 NM_003667 NP_003658.1

Validation

GPR49 / LGR5 Antibody - Brain, Caudate
Brain, Caudate
Brain, Caudate

GPR49 / LGR5 Antibody - Brain, glioblastoma
Brain, glioblastoma
Brain, glioblastoma

GPR49 / LGR5 Antibody - Brain, hippocampus
Brain, hippocampus
Brain, hippocampus

GPR49 / LGR5 Antibody - Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)

GPR49 / LGR5 Antibody - Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

Publications (3)

Expression of the adult intestinal stem cell marker Lgr5 in the metastatic cascade of colorectal cancer. Kleist B, Xu L, Li G, Kersten C. International journal of clinical and experimental pathology. 2011 4:327-35. (IHC-P; Human)

EP receptor expression in human intestinal epithelium and localization relative to the stem cell zone of the crypts. Olsen Hult LT, Kleiveland CR, Fosnes K, Jacobsen M, Lea T. PloS one. 2011 6:e26816. (ICC, IHC-P; Human)

Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses. Chen QQ, Yan L, Wang CZ, Wang WH, Shi H, Su BB, Zeng QH, Du HT, Wan J. World journal of gastroenterology : WJG. 2013 Aug;19:4702-17.

Customer Reviews (0)


Requested From: United States
Date Requested: 12/12/2019