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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B5406-50 50 µg (0.5 mg/ml) $495 
FGF1 / Acidic FGF Antibody - Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
FGF1 / Acidic FGF Antibody - Immunofluorescence of monoclonal antibody to FGF1 on HeLa cell. [antibody concentration 10 ug/ml].
FGF1 / Acidic FGF Antibody - Western blot of FGF1 expression in transfected 293T cell line by FGF1 monoclonal antibody clone 2E12.
FGF1 / Acidic FGF Antibody - Detection limit for recombinant GST tagged FGF1 is approximately 10 ng/ml as a capture antibody.
FGF1 / Acidic FGF Antibody - Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
FGF1 / Acidic FGF Antibody - Immunofluorescence of monoclonal antibody to FGF1 on HeLa cell. [antibody concentration 10 ug/ml].
FGF1 / Acidic FGF Antibody - Western blot of FGF1 expression in transfected 293T cell line by FGF1 monoclonal antibody clone 2E12.
FGF1 / Acidic FGF Antibody - Detection limit for recombinant GST tagged FGF1 is approximately 10 ng/ml as a capture antibody.
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PathPlus™ Monoclonal FGF1 / Acidic FGF Antibody (clone 2E12, IHC, IF, WB) LS‑B5406

PathPlus™ Monoclonal FGF1 / Acidic FGF Antibody (clone 2E12, IHC, IF, WB) LS‑B5406

Note: This antibody replaces LS-C133186
Antibody:
Mouse Monoclonal to Human FGF1 / Acidic FGF
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$495 
LS-B5406-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human FGF1 / Acidic FGF
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FGF1 (Acidic fibroblast growth factor 1) is a member of the fibroblast growth factor family and is involved in embryonic development and organogenesis, morphogenesis, cell growth, tissue repair and tumorigenesis. It regulates endothelial cell migration, proliferation and angiogenesis, and it is involved in learning where it is necessary for memory encoding and enhancement. FGF1 is also an important hormone in metabolic processes including the management of nutrient stress, insulin sensitivity and glycemic control, and it is of interest in injection therapies for type 2 diabetes mellitus. In immunohistochemistry, FGF1 is primarily positive in the cytoplasm of neurons and endothelial cells in the brain, but it also shows positivity in heart muscle, renal tissue, and breast tissue. References: Nat Rev Endocrinol. 2017 Oct;13(10):599-609, PMID: 28664920; Brain Res Bull. 2018 Jul;141:3-12, PMID: 29477835; Neuron. 1991 Sep;7(3):349-64, PMID: 1716928
Target
Human FGF1 / Acidic FGF
Synonyms
FGF1 | AFGF | Endothelial cell growth factor | Fibroblast growth factor 1 | FGFA | ECGFB | HBGF-1 | GLIO703 | ECGF | ECGF-beta | ECGFA | FGF-1 | FGF-alpha | HBGF1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
2E12
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FGF1 (AAH32697, 46 aa ~ 155 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human FGF1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FGF1 / Acidic FGF
P05230 X59065 P05230

Validation

FGF1 / Acidic FGF Antibody - Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

PathPlus™ FGF1 / Acidic FGF Antibody (clone 2E12) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B5406 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 3/28/2020