Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B7722-100 100 µl (1 mg/ml) $254 
TP53 / p53 Antibody - Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
TP53 / p53 Antibody - Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.
TP53 / p53 Antibody - Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using p53 Antibody. The lane on the right is blocked with the synthesized peptide.
TP53 / p53 Antibody - Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
TP53 / p53 Antibody - Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.
TP53 / p53 Antibody - Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using p53 Antibody. The lane on the right is blocked with the synthesized peptide.
1 of 3
2 of 3
3 of 3

PathPlus™ TP53 / p53 Antibody aa283‑332 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B7722

PathPlus™ TP53 / p53 Antibody aa283‑332 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B7722

Note: This antibody replaces LS-C120538
Antibody:
Rabbit Polyclonal to Human TP53 / p53
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$254 
LS-B7722-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TP53 / p53
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TP53 is a tumor suppressor, repair gene and transcriptional regulator, and it is considered the most frequently mutated gene in cancer. Germline mutations in TP53 lead to Li-Fraumeni syndrome and cancer, including early-onset colorectal cancer. Antibodies are used to identify both wild-type and mutant TP53 and its accumulation in various neoplasias, and have strong staining in tumors with mutations that stabilize the protein. TP53 stains positively in breast, lung and endometrial carcinoma, follicular and mantle cell lymphoma, serous ovarian carcinoma, transitional cell carcinoma of the bladder and colorectal adenocarcinoma. TP53 is also often overexpressed in colorectal dysplasia resulting from ulcerative colitis. In normal tissue, TP53 has weak nuclear staining in fractions of cells in most tissues. Genome Res. 2017 Oct;27(10):1645-1657, PMID: 28904012; JAMA Oncol. 2015 May;1(2):214-21, PMID: 26086041; Int J Mol Sci. 2017 Jun 16;18(6). pii: E1284, PMID: 28621756
Target
Human TP53 / p53
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human p53. (AA Range: 283-332)
Epitope
aa283-332
Specificity
This antibody detects endogenous levels of total p53 protein.
Usage
Western Blot: Predicted MW 43 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

Validation

TP53 / p53 Antibody - Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

Publications (1)

Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. Malchenko S, Sredni ST, Bi Y, Margaryan NV, Boyineni J, Mohanam I, Tomita T, Davuluri RV, Soares MB. PloS one. 2017 12:e0173106. (IHC, WB; Mouse)

Customer Reviews (0)


Requested From: United States
Date Requested: 12/10/2019