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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B7722-100 100 µl (1 mg/ml) $425 
TP53 / p53 Antibody - Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
TP53 / p53 Antibody - Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.
TP53 / p53 Antibody - Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using p53 Antibody. The lane on the right is blocked with the synthesized peptide.
TP53 / p53 Antibody - Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
TP53 / p53 Antibody - Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.
TP53 / p53 Antibody - Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using p53 Antibody. The lane on the right is blocked with the synthesized peptide.
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PathPlus™ Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (aa283‑332, IHC, IF, WB) LS‑B7722

PathPlus™ Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (aa283‑332, IHC, IF, WB) LS‑B7722

Note: This antibody replaces LS-C120538
Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (aa283-332) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B7722-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (aa283-332) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TP53 is a tumor suppressor, repair gene and transcriptional regulator, and it is considered the most frequently mutated gene in cancer. Germline mutations in TP53 lead to Li-Fraumeni syndrome and cancer, including early-onset colorectal cancer. Antibodies are used to identify both wild-type and mutant TP53 and its accumulation in various neoplasias, and have strong staining in tumors with mutations that stabilize the protein. TP53 stains positively in breast, lung and endometrial carcinoma, follicular and mantle cell lymphoma, serous ovarian carcinoma, transitional cell carcinoma of the bladder and colorectal adenocarcinoma. TP53 is also often overexpressed in colorectal dysplasia resulting from ulcerative colitis. In normal tissue, TP53 has weak nuclear staining in fractions of cells in most tissues. References: Genome Res. 2017 Oct;27(10):1645-1657, PMID: 28904012; JAMA Oncol. 2015 May;1(2):214-21, PMID: 26086041; Int J Mol Sci. 2017 Jun 16;18(6). pii: E1284, PMID: 28621756
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human p53. (AA Range: 283-332)
Epitope
aa283-332
Specificity
This antibody detects endogenous levels of total p53 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot: Predicted MW 43 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

Validation

TP53 / p53 Antibody - Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

LSBio Ratings

PathPlus™ TP53 / p53 Antibody (aa283-332) for IHC, IF/Immunofluorescence, WB/Western LS-B7722 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. Malchenko S, Sredni ST, Bi Y, Margaryan NV, Boyineni J, Mohanam I, Tomita T, Davuluri RV, Soares MB. PloS one. 2017 12:e0173106. (IHC, WB; Mouse)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/29/2020