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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B4657-100 100 µl $485 
HGF / Hepatocyte Growth Factor Antibody - Anti-HGF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HGF / Hepatocyte Growth Factor Antibody - Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HGF / Hepatocyte Growth Factor Antibody - HGF antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HGF / Hepatocyte Growth Factor Antibody - Anti-HGF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HGF / Hepatocyte Growth Factor Antibody - Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HGF / Hepatocyte Growth Factor Antibody - HGF antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PathPlus™ HGF / Hepatocyte Growth Factor Antibody aa216‑265 Polyclonal IHC,WB LS‑B4657

PathPlus™ HGF / Hepatocyte Growth Factor Antibody aa216‑265 Polyclonal IHC,WB LS‑B4657

Note: This antibody replaces LS-C110933
Antibody:
Rabbit Polyclonal to Human HGF / Hepatocyte Growth Factor
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B4657-100
100 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HGF / Hepatocyte Growth Factor
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
HGF (Hepatocyte growth factor, scatter factor) is a protein secreted by mesenchymal cells that functions in paracrine cell motility, growth and morphogenesis, as well as embryogenesis, organ regeneration and wound healing. It targets epithelial cells, endothelial cells, oligodendrocyte precursor cells, hameopoietic progenitor cells and T cells to induce migration as well as proliferative and angiogenic processes. Levels of expression of HGF are positively correlated with number of neocortical GABAergic interneurons and decreased HGF levels are associated with susceptibility to seizures -- increasing HGF levels has been shown to help lower susceptibility. HGF can activate Schwann cells to promote peripheral nerve regeneration. It is also thought to be involved in atherogenesis and is found in atherosclerotic plaques. In immunohistochemistry, HGF has moderate extracellular and cytoplasmic positivity in most tissues throughout the body. References: Biomed Res Int. 2013;2013:470418, PMID: 24490163; EXS. 1993;65:225-49, PMID: 8422545; Exp Cell Res. 2001 Jul 15;267(2):258-66, PMID: 11426944; Atherosclerosis. 2002 Sep;164(1):79-87; Atherosclerosis. 2002 Sep;164(1):79-87, PMID: 12119196; Scientific Reports. 2018. 8 (8316), DOI: 10.1038/s41598-018-26704-x; J Neurosci Res. 2002 Sep 1;69(5):597-606, PMID: 12210825;
Target
Human HGF / Hepatocyte Growth Factor
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa216-265 of human HGF (P14210, NP_000592). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Bat (92%); Turkey, Zebra finch, Chicken (85%).
Blocking Peptide
LS-E16280 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4657. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa216-265
Specificity
Human HGF
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HGF / Hepatocyte Growth Factor
P14210 X16323 CAA34387.1

Validation

HGF / Hepatocyte Growth Factor Antibody - Anti-HGF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HGF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HGF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

HGF / Hepatocyte Growth Factor Antibody - Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (1)

Novel MET/TIE2/VEGFR2 inhibitor altiratinib inhibits tumor growth and invasiveness in bevacizumab-resistant glioblastoma mouse models. Piao Y, Park SY, Henry V, Smith BD, Tiao N, Flynn DL, de Groot JF. Neuro-oncology. 2016 September;18:1230-41. (Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 1/22/2020