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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B7173-50 50 µg (1 mg/ml) $425 
CEA / Carcinoembryonic Antigen Antibody - Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PathPlus™ CEA / Carcinoembryonic Antigen Antibody Monoclonal Flo,ICC,IHC,WB LS‑B7173

PathPlus™ CEA / Carcinoembryonic Antigen Antibody Monoclonal Flo,ICC,IHC,WB LS‑B7173

Note: This antibody replaces LS-C146735
Antibody:
Mouse Monoclonal to Human CEA / Carcinoembryonic Antigen
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7173-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human CEA / Carcinoembryonic Antigen
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Carcinoembryonic (CEA / mCEA / pCEA) antigen includes a large class of proteins such as CEACAM5 that are have high expression in a variety of cancers. The degree of expression of CEA is correlated with tumor stage, with particularly pronounced levels in well-differentiated adenocarcinomas relative to early tumors. Staining is observed in normal epithelial cells of the colon, granulocytes, gallbladder, hepatocytes, prostate, and small intestine goblet cells, as well as thyroid and C cells. Positive staining has been observed in cancers of the bile duct, bladder, cervix, cholangiocarcinoma, colorectal cancer, endometrium, stomach, liver, lung adenocarcinomas, ovary, pancreas, salivary gland, medullary thyroid carcinoma, and others. Staining is expected to be membranous and cytoplasmic. Experimental Cell Research 324. 2014 June. (2):115–23, DOI:10.1016/j.yexcr.2014.04.007; Current Oncology (Toronto, Ont.). 2014 Feb. 21 (1):e1-7, PMID: 24523606; Med Arch. 2013 Dec;67(6):397-401, PMID: 25568506
Target
Human CEA / Carcinoembryonic Antigen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
PARLAM 4
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:25 - 1:100)
  • ICC
  • Western blot (1:100 - 1:500)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Isolated human CEA from human colonic carcinoma cells
Specificity
Most polyclonal CEA antisera show cross-reactivity with related antigens such as biliary glycoprotein (BGP) and non-specific cross-reacting antigen 1/11 (NCA). Does not show cross reactivity, neither with BGP nor with NCA. In immunoblotting the antibody recognizes a single band of 180 kD.
Usage
Methanol fixed cells are recommended for immunocytochemistry. A PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended for immunohistochemistry on frozen tissues.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

CEA / Carcinoembryonic Antigen Antibody - Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Requested From: United States
Date Requested: 12/10/2019