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Catalog Number Size Price
LS-B12051-0.05 0.05 mg (1 mg/ml) $425 
APOBEC3B Antibody - Human Breast Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus™ Polyclonal APOBEC3B Antibody (IHC, IF, WB) LS‑B12051

PathPlus™ Polyclonal APOBEC3B Antibody (IHC, IF, WB) LS‑B12051

Note: This antibody replaces LS-C336146
Antibody:
Rabbit Polyclonal to Human APOBEC3B
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B12051-0.05
0.05 mg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human APOBEC3B
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
APOBEC3B is a repair gene and cytidine deaminase involved in somatic hypermutation. The deregulation or dysfunction of APOBEC3B and other members of the APOBEC family has been implicated as a direct source of mutagenesis in breast and other cancers. APOBEC3B is upregulated in a number of breast cancers, and this upregulation correlates with a mutation spectrum (kataegis) in these tumors consistent with APOBEC function and may be a simultaneous or upstream consequence of ERBB2 amplification. APOBEC mutation signatures have also been identified in lung cancer, as well as evidence of APOBEC3B upregulation in lung cancer and a correlation between increased expression and worse prognosis in non-small-cell lung carcinoma (NSCLC), making it an important potential biomarker for the disease. In immunohistochemistry, APOBEC3B has low levels of nuclear positivity in tissues throughout the body, with highest expression in peripheral blood leukocytes and in the spleen, heart, thymus, prostate, ovary, and testes. References: Genome Biol. 2016; 17: 185, PMID: 27634334; eLife. 2013; 2: e00534, PMID: 23599896; Oncogene. 2018 Jul;37(29):3924-3936, PMID: 29695832; Elife. 2013 Apr 16;2:e00534. doi: 10.7554/eLife.00534, PMID: 23599896; Br J Cancer. 2017 Jun 27; 117(1): 113–123, PMID: 28535155;
Target
Human APOBEC3B
Synonyms
APOBEC3B | APOBEC1L | ARCD3 | BK150C2.2 | Cytidine deaminase | DJ742C19.2 | Phorbolin 2 | Phorbolin-1-related protein | PHRBNL | ARP4 | Phorbolin 3 | Phorbolin-2/3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC - Paraffin (5 - 10 µg/ml)
  • Immunofluorescence (20 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 14 AA peptide near the N-Terminus of human APOBEC3B. The immunogen is located within aa 20-70 of APOBEC3B.
Blocking Peptide
LS-E45922 - Liquid - 0.05 mg 200 µg/ml - $145.00
Immunizing peptide used to generate LS-B12051. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist; this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D.
Usage
APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 42 kDa Observed: 48 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOBEC3B
Q9UH17 NM_004900 NP_004891.4

Validation

APOBEC3B Antibody - Human Breast Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Requested From: United States
Date Requested: 2/23/2020