Features: - Blue-gray chromogen precipitant - Precipitated by Peroxidase - Suitable for aqueous (hardening) and non-aqueous (permanent) mounting - Sufficient reagents to produce 300 ml of working solution - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, ISH, Blotting Applications, and Elispot - Suitable for use in single or multiple labeling assays - Additional substrate properties contrast with tissue pigments - No wait times for mixing and dissolving powders or tablets - One year expiry date Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot Details: Vector® SG and ImmPACT™ SG HRP substrates produce blue-gray reaction products that can be used as a single label or as a second color for multiple antigen labeling. With the aid of imaging systems and software, the spectral profile of both chromogens can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield or electron microscopy. Vector® SG and ImmPACT™ SG substrates can be used for both manual and automated staining methods. Stained sections can be dehydrated, cleared, and permanently mounted. Vector® SG Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector® SG substrate is equivalent to AEC. This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 °C, this kit is stable for one year.