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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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VECTASHIELD HardSet Antifade Mounting Medium with DAPI - LS-J1035
Available for shipment within the USA only
Catalog
Size
Price
LS-J1035-10
10 ml
$180

Available for shipment within the USA only

Product Description

A ready-to-use aqueous mounting medium that hardens for long term storage without sealing. Inhibits photobleaching of fluorescent dyes and proteins, and contains DAPI counterstain.

Specifications

Details
Features: - Ready-to-use aqueous solution - Hardening formulation - Contains DAPI counterstain (blue fluorescence staining of nuclei) - Inhibits photobleaching of fluorescent dyes and fluorescent proteins - No warming necessary - No sealing necessary for long term storage - Ideal refractive index (1.46) - 10 ml volume - Six month expiration date Applications: - Immunofluorescence - In situ hybridization - Cellular Imaging - Super-Resolution Microscopy Details: VECTASHIELD HardSet Antifade Mounting Medium with DAPI preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. This solution contains DAPI (4 , 6-diamidino-2-phenylindole), which produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm. The DAPI concentration can be modified by mixing with VECTASHIELD HardSet Antifade Mounting Medium without DAPI (LS-F1034). VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. See the VECTASHIELD® Mounting Media Compatibility document to determine if VECTASHIELD® will be compatible in your system. Antifade Comparison: Other manufacturers measure the antifade properties of their mounts using labeled microspheres or arrayed spots. Vector® Labs prefers to measure antifade properties of VECTASHIELD® mounts using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.
Restrictions
For research use only. Available for shipment within the USA only.

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Images

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.
Astrocytes: Stained for GFAP and detected with DyLight® 488 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 549 labeled secondary antibody (red). S100, DyLight® 488 labeled secondary antibody (green). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Immunofluorescence

Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight® 488 labeled secondary antibody (green). S100, DyLight® 594 labeled secondary antibody (red). Mounted in VECTASHIELD® HardSet Mounting Medium with DAPI.

Requested From: United States
Date Requested: 12/13/2018
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