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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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VECTASHIELD HardSet Antifade Mounting Medium - LS-J1034

Available for shipment within the USA only
Catalog
Size
Price
LS-J1034-10
10 ml
$180

Available for shipment within the USA only

Product Description

A ready-to-use aqueous mounting medium that hardens for long term storage without sealing. Inhibits photobleaching of fluorescent dyes and proteins, and contains no counterstain.

Specifications

Details
Features: - Ready-to-use aqueous solution - Contains no counterstain - Hardening formulation - Inhibits photobleaching of fluorescent dyes and fluorescent proteins - No warming necessary - No sealing necessary for long term storage - Contains no counterstain - Ideal refractive index (1.46) - 10 ml volume - Six month expiration date Applications: - Immunofluorescence - In situ hybridization - Cellular Imaging - Super-Resolution Microscopy Details: VECTASHIELD HardSet Antifade Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. This product can be used to dilute VECTASHIELD Hardset Antifade Mounting Medium with DAPI (LS-J1035) or VECTASHIELD HardSet Antifade Mounting Medium with Phalloidin (LS-J1036) to adjust the DAPI or Phalloidin concentrations. VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. See the VECTASHIELD® Mounting Media Compatibility document to determine if VECTASHIELD® will be compatible in your system. Antifade Comparison: Other manufacturers measure the antifade properties of their mounts using labeled microspheres or arrayed spots. Vector® Labs prefers to measure antifade properties of VECTASHIELD® mounts using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.
Restrictions
For research use only. Available for shipment within the USA only.

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Images

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.
C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.

Immunofluorescence

Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.
Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.
C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.

Immunofluorescence

Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.
Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.
C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.

Immunofluorescence

Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.
Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight® 594 Goat Anti-Rabbit IgG; plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight® 488 Streptavidin.

Immunofluorescence

C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.
C6 rat glioma cells: Antibody to mitochondria localized with Alexa 555 labeled secondary antibody. Mounted in VECTASHIELD® HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.

Immunofluorescence

Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.
Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD® HardSet Mounting Medium.

Requested From: United States
Date Requested: 3/26/2019
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