Features: - 2 to 4 fold greater sensitivity than original substrate kit - Red chromogen precipitant - Precipitated by Peroxidase - Suitable for aqueous mounting (hardening and non-hardening) - For used on nylon or PVDF membranes but is not recommended for nitrocellulose membranes - Supplied as 120 ml of diluent and stock solutions in dropper bottles - For brightfield and spectral imaging - Greater sensitivity than conventional substrates - Suitable for IHC, ICC, ISH, Blotting Applications, and Elispot - Suitable for use in single or multiple labeling assays - Stock solutions supplied in convenient dropper bottles promoting ease of handling - No wait times for mixing and dissolving powders or tablets - Working solution stable for up to 14 days at 4 °C - One year expiry date Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot Details: AEC (3-amino-9-ethylcarbazole) and ImmPACT™ AEC HRP Substrates both produce red reaction products that can be used as a single label or as a second color for multiple antigen labeling. AEC and ImmPACT™ AEC chromogens can be used for both manual and automated staining methods. Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents. Sections stained with AEC are stable for at least 4 years when mounted in VectaMount™ AQ (LS-J1038). This product utilizes Vector® Laboratories novel ImmPACT™ technology to generate a crisper, brighter red reaction product that is 2-4 times more sensitive than conventional AEC substrates such as AEC Substrate Kit (LS-J1076) This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT™ AEC reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 47° C.