Sample diluents are used to dilute samples into the functional range of the assay and to create the standard curve. Due to the finite binding capacity of the plate well-coated proteins (e.g., antibodies, antigens), highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument. Properly formulated sample diluents will also reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate well surface. Protein-Free Sample Diluent provides a non-protein-based buffered, neutral pH environment that is highly compatible with antibody-antigen interactions. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C. • Clear liquid • 1X ready to use • pH 7.1-7.6

Recommended Storage: Store at 2-8°C.