Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
All of the pCMV vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
Hygromycin resistance gene for selection in mammalian cell lines.
A Kozak consensus sequence to enhance mammalian expression.
CMV promoter: bp 250-837 Enhancer: bp 838-1445 SV40 early promoter: bp 2399-2768 Hygromycin ORF: bp 2786-3811 pUC origin: bp 4454-5127 Kanamycin ORF: bp 5201-6016
DDK (Flag)-tag, or DDK (Flag) octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed proteins from wild-type proteins expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A DDK (Flag)-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a DDK (Flag)-tag to this protein allows one to follow the protein with an antibody against the DDK (Flag) sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. The peptide sequence of the DDK (Flag)-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine-tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g. M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g. M2) are position-insensitive.