All of the pCMV vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
- Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
- Hygromycin resistance gene for selection in mammalian cell lines.
- A Kozak consensus sequence to enhance mammalian expression.
CMV promoter: bp 250-837
Enhancer: bp 838-1445
SV40 early promoter: bp 3047-3416
Hygromycin ORF: bp 3932-3930
pUC origin: bp 5102-5775
Kanamycin ORF: bp 5849-6664