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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Rat MAP2K1 / MKK1 / MEK1 ELISA Kit (Sandwich ELISA) - LS-F39033
LS-F39033 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat MAP2K1 / MKK1 / MEK1 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of MAP2K1 / MKK1 / MEK1 as low as 0.063 nanograms per millilter.
Catalog
Size
Price
LS-F39033-1
1 plate
$720
Available Discounts
10% off 5+ units

Popular MAP2K1 / MKK1 / MEK1 Elisa Kits

Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Tissue Homogenates

Product Description

LS-F39033 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat MAP2K1 / MKK1 / MEK1 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of MAP2K1 / MKK1 / MEK1 as low as 0.063 nanograms per millilter.
About MAP2K1 / MKK1 / MEK1
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Q02750 NM_002755 NP_002746.1

MAP2K1 ElisaKit, ERK activator kinase 1 ElisaKit, MAPK/ERK kinase 1 ElisaKit, MAPKK 1 ElisaKit, MKK1 ElisaKit, PRKMK1 ElisaKit, MEK 1 ElisaKit, MAP kinase kinase 1 ElisaKit, MAPKK1 ElisaKit, MEK1 ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
MAP2K1 / MKK1 / MEK1
Reactivity
Rat
Manual
Intended Sample Types
Cell Lysates, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.063
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Adhesive Plate Sealers
  • Sample Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Wash Buffer (30x)
  • TMB Substrate
  • Stop Solution
  • Standard (Lyophilized)
  • Detection Reagent A (Green)
  • Detection Reagent B (Red)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/21/2018

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