Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
LS-F23902 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat FNDC5 / Irisin in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of FNDC5 / Irisin as low as 37.5 picograms per milliliter.
This kit recognizes natural and recombinant Rat FNDC5.
Intended Sample Types
Colorimetric - 450nm (TMB)
62.5 - 4000 pg/ml
Intra-Assay: CV<5.26% Inter-Assay: CV<5.13%
Short term: 4°C; Long term: see manual.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Adhesive Plate Sealers
Prepare all reagents, samples and standards.
Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
Aspirate and wash 3 times.
Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
Aspirate and wash 5 times.
Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.