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Rat CASP8AP2 / FLASH ELISA Kit (Sandwich ELISA) - LS-F9528
Caspase 8 Associated Protein 2 (CASP8AP2)
No significant cross-reactivity or interference between Caspase 8 Associated Protein 2 (CASP8AP2) and analogs was observed.
LS-F9528 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Rat CASP8AP2 / FLASH in samples of Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CASP8AP2 / FLASH as low as 0.136 nanograms per millilter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
0.313 - 20 ng/ml
0.136 ng/ml
4.5 h
Intra-Assay: CV<10% Inter-Assay: CV<12%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1).
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