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Rat BCL3 / BCL-3 ELISA Kit (Sandwich ELISA) - LS-F36222

Catalog Size Price
LS-F36222-1 1 plate Unavailable

Most Popular BCL3 / BCL-3 Elisa Kits

Human BCL3 / BCL-3 ELISA Kit (Sandwich ELISA) - LS-F10924
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96-Well Strip Plate
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0.156-10 ng/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Mouse BCL3 / BCL-3 ELISA Kit (Custom ELISA) - LS-F18397
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Rat BCL3 / BCL-3 ELISA Kit (Sandwich ELISA) - LS-F23948
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Rat BCL3 / BCL-3 ELISA Kit (CLIA) - LS-F31117
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Rat BCL3 / BCL-3 ELISA Kit (Sandwich ELISA) - LS-F36222
Quantitative Sandwich ELISA
96-Well Strip Plate
Rat
6.25-400 pg/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma

100% Guaranteed
LS-F36222
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
BCL3 / BCL-3
Rat
LS-F36222 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat BCL3 / BCL-3 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of BCL3 / BCL-3 as low as 3.75 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
6.25 - 400 pg/ml
3.75
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BCL-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BCL-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BCL-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BCL-3 can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About BCL3 / BCL-3

P20749 NM_005178 NP_005169.2

BCL3 ElisaKit, B-cell CLL/lymphoma 3 ElisaKit, B-cell leukemia/lymphoma 3 ElisaKit, B-cell lymphoma 3 protein ElisaKit, BCL-3 ElisaKit, BCL4 ElisaKit, D19S37 ElisaKit, Proto-oncogene BCL3 ElisaKit

BCL3 / BCL-3 is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/27/2018

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