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Rat AQP2 / Aquaporin 2 ELISA Kit (Competitive EIA) - LS-F39189

Catalog Size Price
LS-F39189-1 1 plate Unavailable

Most Popular AQP2 / Aquaporin 2 Elisa Kits

Human AQP2 / Aquaporin 2 ELISA Kit (Sandwich ELISA) - LS-F4051
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Rat AQP2 / Aquaporin 2 ELISA Kit (Sandwich ELISA) - LS-F14835
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Mouse AQP2 / Aquaporin 2 ELISA Kit (Sandwich ELISA) - LS-F20747
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100% Guaranteed
LS-F39189
Competitive EIA (enzyme immunoassay) kit
AQP2 / Aquaporin 2
Rat
LS-F39189 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat AQP2 / Aquaporin 2 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of AQP2 / Aquaporin 2 as low as 0.938 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
1.563 - 100 ng/ml
0.938
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with AQP-2. During the reaction, AQP-2 in the sample or standard competes with a fixed amount of AQP-2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to AQP-2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AQP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Short term: 4°C; Long term: see manual.
For research use only.

About AQP2 / Aquaporin 2

P41181 NM_000486 NP_000477.1

AQP2 ElisaKit, AQP-CD ElisaKit, ADH water channel ElisaKit, Aquaporin 2 (collecting duct) ElisaKit, AQP-2 ElisaKit, Aquaporin 2 ElisaKit, Aquaporin-CD ElisaKit, Aquaporin-2 ElisaKit, Water-channel aquaporin 2 ElisaKit, WCH-CD ElisaKit

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

 

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: 
Date Requested: 5/27/2018

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