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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Rat Apelin ELISA Kit (Competitive EIA) - LS-F39393
LS-F39393 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Apelin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Apelin as low as 46.875 picograms per milliliter.
Catalog
Size
Price
LS-F39393-1
1 plate
$550

Popular Apelin Elisa Kits

Type: Competitive EIA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 19.75-1600 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Competitive EIA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 12.35-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Competitive EIA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Range: 0.1-1000 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 78-5000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Competitive EIA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 125-8000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F39393 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Apelin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Apelin as low as 46.875 picograms per milliliter.
About Apelin
Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Q9ULZ1 NM_017413 NP_059109.3

APLN ElisaKit, AGTRL1 ligand ElisaKit, Apelin ElisaKit, APEL ElisaKit, Preproapelin ElisaKit, APJ endogenous ligand ElisaKit, XNPEP2 ElisaKit


Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
Apelin
Reactivity
Rat
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
78.125 - 5000 pg/ml
Sensitivity
46.875
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
Background
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Apln. During the reaction, Apln in the sample or standard competes with a fixed amount of Apln on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Apln. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Apln in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: United States
Date Requested: 8/17/2018

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