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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Rat ALOX5 / 5-LOX ELISA Kit (Sandwich ELISA) - LS-F36217
LS-F36217 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat ALOX5 / 5-LOX in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of ALOX5 / 5-LOX as low as 0.094 nanograms per millilter.
Catalog
Size
Price
LS-F36217-1
1 plate
$790

Popular ALOX5 / 5-LOX Elisa Kits

Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.781-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures

Product Description

LS-F36217 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat ALOX5 / 5-LOX in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of ALOX5 / 5-LOX as low as 0.094 nanograms per millilter.
About ALOX5 / 5-LOX
ALOX5 / 5-LOX is a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). P09917 NM_000698 NP_000689.1

ALOX5 ElisaKit, 5-lipoxygenase ElisaKit, 5LPG ElisaKit, 5-LO ElisaKit, 5-LOX ElisaKit, Arachidonate 5-lipoxygenase ElisaKit, LOG5 ElisaKit, LOX-5 ElisaKit, Leukotriene A4 synthase ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
ALOX5 / 5-LOX
Reactivity
Rat
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.094
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- 5-LO antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-5-LO antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a b lue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 5-LO amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of 5-LO can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/19/2018

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