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Rat ALOX5 / 5-LOX ELISA Kit (Sandwich ELISA) - LS-F36217

Catalog Size Price
LS-F36217-1 1 plate Unavailable

Most Popular ALOX5 / 5-LOX Elisa Kits

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100% Guaranteed
LS-F36217
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
ALOX5 / 5-LOX
Rat
LS-F36217 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat ALOX5 / 5-LOX in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of ALOX5 / 5-LOX as low as 0.094 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.156 - 10 ng/ml
0.094
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- 5-LO antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-5-LO antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a b lue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 5-LO amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of 5-LO can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About ALOX5 / 5-LOX

P09917 NM_000698 NP_000689.1

ALOX5 ElisaKit, 5-lipoxygenase ElisaKit, 5LPG ElisaKit, 5-LO ElisaKit, 5-LOX ElisaKit, Arachidonate 5-lipoxygenase ElisaKit, LOG5 ElisaKit, LOX-5 ElisaKit, Leukotriene A4 synthase ElisaKit

ALOX5 / 5-LOX is a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4).

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/25/2018

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