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Rabbit NPPA / ANP ELISA Kit (Sandwich ELISA) - LS-F36219

Catalog Size Price
LS-F36219-1 1 plate Unavailable

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LS-F36219
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
NPPA / ANP
Rabbit
LS-F36219 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rabbit NPPA / ANP in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of NPPA / ANP as low as 9.375 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
15.625 - 1000 pg/ml
9.375
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ANP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ANP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blu e color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANP can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About NPPA / ANP

P01160 NM_006172 NP_006163.1

NPPA ElisaKit, ANF ElisaKit, ANP ElisaKit, Atriopeptin ElisaKit, ATFB6 ElisaKit, Atrial natriuretic factor ElisaKit, CDD-ANF ElisaKit, Cardionatrin ElisaKit, Natriuretic peptides A ElisaKit, Prepronatriodilatin ElisaKit, Natriuretic peptide A ElisaKit, PND ElisaKit

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/24/2018

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