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Oxytocin ELISA Kit (Competitive EIA) - LS-F39451

Catalog Size Price
LS-F39451-1 1 plate Unavailable

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100% Guaranteed
LS-F39451
Competitive EIA (enzyme immunoassay) kit
Oxytocin
All species
LS-F39451 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Oxytocin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Oxytocin as low as 9.375 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
15.625 - 1000 pg/ml
9.375
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with OT. During the reaction, OT in the sample or standard competes with a fixed amount of OT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to OT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Short term: 4°C; Long term: see manual.
For research use only.

About Oxytocin

P01178 NM_000915 NP_000906.1

OXT ElisaKit, OXT-NPI ElisaKit, Oxytocin ElisaKit, OT ElisaKit, Oxytocin, prepropeptide ElisaKit, Oxytocin-neurophysin 1 ElisaKit, Neurophysin I ElisaKit, OT-NPI ElisaKit

Oxytocin is a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream.

 

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: 
Date Requested: 5/22/2018

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