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Mouse NOS2 / iNOS ELISA Kit (Sandwich ELISA) - LS-F36660
LS-F36660 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse NOS2 / iNOS in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of NOS2 / iNOS as low as 0.188 nanograms per millilter.
Catalog
Size
Price
LS-F36660-1
1 plate
$790

Popular NOS2 / iNOS Elisa Kits

Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 15.6-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 1.56-100 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Tissue Homogenates
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 15.6-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Human
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.312-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F36660 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse NOS2 / iNOS in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of NOS2 / iNOS as low as 0.188 nanograms per millilter.
About NOS2 / iNOS
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. P35228 NM_000625 NP_000616.3

NOS2 ElisaKit, Hepatocyte NOS ElisaKit, HEP-NOS ElisaKit, Inducible NOS ElisaKit, NOS, type II ElisaKit, NOS type II ElisaKit, Inducible NO synthase ElisaKit, NOS2A ElisaKit, INOS ElisaKit, NOS ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
NOS2 / iNOS
Reactivity
Mouse
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 ng/ml
Sensitivity
0.188
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NOS2/iNOS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NOS2/iNOS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NOS2/iNOS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofNOS2/iNOS can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/21/2018

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