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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse IL12B / IL12 p40 ELISA Kit (Sandwich ELISA) - LS-F2767
Catalog
Size
Price
LS-F2767-1
1 plate
$490

Popular IL12B / IL12 p40 Elisa Kits

Reactivity: Bovine
Range: 78-5000 pg/ml
Reactivity: Dog
Range: 15.63-1000 pg/ml
Reactivity: Rat
Range: 15.63-1000 pg/ml
Reactivity: Human
Range: 7.81-500 pg/ml

Product Description

LS-F2767 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IL12B / IL12 p40 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL12B / IL12 p40 as low as 10 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL12B / IL12 p40
Synonyms
IL12B, CLMF p40, IL-12 p40, IL-12 subunit p40, Interleukin-12 beta chain, Interleukin-12 subunit beta, IL12, subunit p40, Interleukinl-12, Interleukin 12, p40, NKSF, CLMF, CLMF2, IL-12B, NKSF2
Reactivity
Mouse
Specificity
This kit is for the detection of natural and recombinant mouse IL-12(p40). No significant cross-reactivity or interference between IL-12(p40) and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
15.6 - 1000 pg/ml
Sensitivity
10 pg/ml
Precision
Intra-Assay: CV%<6.1% Inter-Assay: CV%<7%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • ABC Complex (100x)
  • Sample Diluent
  • Detection Antibody Diluent
  • ABC Complex Diluent
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Restrictions
For research use only.
About IL12B / IL12 p40
IL12B / IL12 p40 is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. P29460 NM_002187 NP_002178.2


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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/17/2018

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