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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse IGFBP3 ELISA Kit (Sandwich ELISA) - LS-F36617
Catalog
Size
Price
LS-F36617-1
1 plate
$790

Popular IGFBP3 Elisa Kits

Reactivity: Rat
Range: 0.156-10 ng/ml
Reactivity: Rat
Range: 0.313-20 ng/ml
Reactivity: Human
Range: 156.2-10000 pg/ml
Reactivity: Human
Range: 74-18000 pg/ml
Reactivity: Mouse
Range: 54.87-40000 pg/ml

Product Description

LS-F36617 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IGFBP3 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IGFBP3 as low as 37.5 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IGFBP3
Synonyms
IGFBP3, Binding protein 29, Binding protein 53, BP-53, IGF-binding protein 3, IGFBP-3, IBP-3, IBP3
Reactivity
Mouse
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
62.5 - 4000 pg/ml
Sensitivity
37.5 pg/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IGFBP-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGFBP-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofIGFBP-3 can be calculated.
Restrictions
For research use only.
About IGFBP3
IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. P17936 NM_000598 NP_000589.2


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Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/23/2018

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