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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat SIRPA / CD172a ELISA Kit (Cell-Based ELISA) - LS-F2308
Catalog
Size
Price
LS-F2308-1
1 plate
$490

Popular SIRPA / CD172a Elisa Kits


Product Description

LS-F2308 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human SIRPA / CD172a in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.

Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
SIRPA / CD172a
Synonyms
SIRPA, CD172A, CD172a antigen, Inhibitory receptors shps-1, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, p84, SHPS-1, SHPS1, SIRP, Sirp-alpha-1, Sirp-alpha-3, Sirp-alpha1, MYD1, SIRPalpha, SIRPalpha2, BIT, MFR, MYD-1, PTPNS1, SHP substrate 1, Sirp-alpha-2
Reactivity
Mouse, Human, Rat
Specificity
Sirp alpha1
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 15x Wash Buffer
  • 100x Anti-Sirp a1 Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                About SIRPA / CD172a
                CD172A / SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. P78324 NM_080792 NP_542970.1


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                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Requested From: United States
                Date Requested: 10/15/2018

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