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Mouse/Human/Rat PNPLA3 / Adiponutrin ELISA Kit (Competitive EIA) - LS-F410

Catalog
Size
Price
LS-F410-1
1 plate
$440
Available Discounts
10% off 2-4 units
20% off 5+ units

Product Description

LS-F410 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human PNPLA3 / Adiponutrin in samples of Cell Culture Supernatants and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of PNPLA3 / Adiponutrin as low as 0.1 nanograms per millilter.

Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
PNPLA3 / Adiponutrin
Reactivity
Mouse, Human, Rat
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Manual
Intended Sample Types
Cell Culture Supernatants, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.1 - 1000 ng/ml
Sensitivity
0.1 ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Microplate
  • Detection Reagents
  • Wash Buffer
  • Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl anti-Adiponutrin antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4°C.
  3. Add 100 µl standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
  4. Add 100 µl prepared streptavidin solution. Incubate 45 minutes at room temperature.
  5. Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
  6. Add 50 µl Stop Solution to each well. Read at 450 nm immediately
Restrictions
For research use only.
About PNPLA3 / Adiponutrin
Q9NST1 NM_025225 NP_079501.2

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Competition ELISA Platform Overview

Requested From: United States
Date Requested: 9/22/2019