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Mouse/Human/Rat Phospho-RB1 / Retinoblastoma / RB (Ser780) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1610
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Technical Help
Review This ProductPopular RB1 / Retinoblastoma / RB Elisa Kits
Type:
Quantitative Sandwich ELISA
Format:
96-Well Strip Plate
Reactivity:
Human
Range:
0.156-10 ng/ml
Detection:
Colorimetric - 450nm (TMB)
Sample Types:
Cell Lysates, Tissue Homogenates
Type:
Qualitative Cell-Based Phosphorylation ELISA
Format:
96-Well Microplate
Reactivity:
Mouse, Human, Rat
Range:
Positive/Negative
Detection:
Colorimetric - 450nm (TMB)
Sample Types:
Adherent Cell Cultures
Type:
Qualitative Cell-Based Phosphorylation ELISA
Format:
96-Well Microplate
Reactivity:
Mouse, Human, Rat
Range:
Positive/Negative
Detection:
Colorimetric - 450nm (TMB)
Sample Types:
Adherent Cell Cultures
Type:
Qualitative Cell-Based Phosphorylation ELISA
Format:
96-Well Microplate
Reactivity:
Mouse, Human, Rat
Range:
Positive/Negative
Detection:
Colorimetric - 450nm (TMB)
Sample Types:
Adherent Cell Cultures
Product Description
LS-F1610 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human RB1 / Retinoblastoma / RB in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
About RB1 / Retinoblastoma / RB
Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest.
P06400
NM_000321
NP_000312.2
RB1 ElisaKit, p105-Rb ElisaKit, Osteosarcoma ElisaKit, PRb ElisaKit, Retinoblastoma ElisaKit, Retinoblastoma 1 ElisaKit, OSRC ElisaKit, RB ElisaKit, Pp110 ElisaKit
Specifications
Type
Cell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
RB1 / Retinoblastoma / RB
Reactivity
Mouse, Human, Rat
Specificity
Retinoblastoma (Phospho-Ser780)
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well Cell Culture Clear-Bottom Microplate
- 10x TBS
- Quenching Buffer
- Blocking Buffer
- 10x Wash Buffer
- 100x Anti-Retinoblastoma (Phospho- Ser780) Antibody (Rabbit Polyclonal)
- 100x Anti-Retinoblastoma Antibody (Rabbit Polyclonal)
- 100x Anti-GAPDH Antibody (Mouse Monoclonal)
- HRP-Conjugated Anti-Rabbit IgG Antibody
- HRP-Conjugated Anti-Mouse IgG Antibody
- Primary Antibody Diluent
- Ready-to-Use Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Seals
Protocol
Restrictions
For research use only.
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